urmjУральский медицинский журналUral Medical Journal2949-4389Ural State Medical University10.52420/2071-5943-2023-22-1-41-50urmj-1190Research ArticleОригинальные статьи | Original articlesOriginal articlesОценка иммунного статуса женщин после процедуры абляционного фракционного лазерного фототермолиза, проведенной с целью коррекции инволюционных изменений кожи лицаAssessment of the immune status of women after ablative fractional laser photothermolysis procedure for the correct of involutional facial skin changeshttps://orcid.org/0000-0001-8670-2828КузнецоваЕ. К.KuznetsovaE. K.

Евгения Константиновна Кузнецова – кандидат медицинских наук 

Оренбург

Evgenia K. Kuznetsova – Ph.D. in medicine 

Orenburg

estroukova@yandex.ruhttps://orcid.org/0000-0001-9155-2334МезенцеваЕ. А.MezentsevaE. A.

Елена Анатольевна Мезенцева – кандидат медицинских наук 

Челябинск

Elena A. Mezentseva – Ph.D. in medicine 

Chelyabinsk

alena_mez_75@mail.ruhttps://orcid.org/0000-0002-8867-0775КудревичЮ. В.KudrevichY. V.

Юлия Валерьевна Кудревич – кандидат медицинских наук 

Челябинск

Yulia V. Kudrevich – Ph.D. in medicine 

Chelyabinsk

cyton@mail.ruhttps://orcid.org/0000-0002-0901-8042ДолгушинИ. И.DolgushinI. I.

Илья Ильич Долгушин – доктор медицинских наук, профессор 

Челябинск

Ilya I. Dolgushin – Doctor of Science (Medicine), Professor 

Chelyabinsk

dol-ii@mail.ruhttps://orcid.org/0000-0002-5857-0319ЗиганшинО. Р.ZiganshinO. R.

Олег Раисович Зиганшин – доктор медицинских наук, профессор 

Челябинск

Oleg R. Ziganshin – Doctor of Science (Medicine), Professor 

Chelyabinsk

ziganshin_oleg@mail.ruhttps://orcid.org/0000-0002-3900-9278НикушкинаК. В.NikushkinaK. V.

Карина Викторовна Никушкина – кандидат медицинских наук 

Челябинск

Karina V. Nikushkina – Ph.D. in medicine 

Chelyabinsk

knikushkina81@gmail.comОренбургский государственный медицинский университетРоссияOrenburg State Medical UniversityRussian FederationЮжно-Уральский государственный медицинский университетРоссияSouth Ural State Medical UniversityRussian Federation2023150320232214150Copyright © Кузнецова Е.К., Мезенцева Е.А., Кудревич Ю.В., Долгушин И.И., Зиганшин О.Р., Никушкина К.В., 20232023Кузнецова Е.К., Мезенцева Е.А., Кудревич Ю.В., Долгушин И.И., Зиганшин О.Р., Никушкина К.В.Kuznetsova E.K., Mezentseva E.A., Kudrevich Y.V., Dolgushin I.I., Ziganshin O.R., Nikushkina K.V.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://www.umjusmu.ru/jour/article/view/1190

Введение. В основе абляционного фракционного лазерного фототермолиза (А-ФЛФ), используемого для омоложения стареющей кожи, лежит ее контролируемое повреждение. В запускаемых процессах репаративной регенерации принимают участие факторы иммунной системы, которая, в свою очередь, также подвергается возрастному ремоделированию или иммуностарению. Цель работы – оценить в динамике реакцию иммунной системы на процедуру А-ФЛФ, проведенную для коррекции возраст-ассоциированных изменений кожи лица. Материалы и методы. В исследование включены 25 женщин от 42 до 55 лет, которым был проведена процедура А-ФЛФ кожи лица с помощью эрбиевого лазера. До процедуры, на 8-е и 24-е сутки после нее в периферической крови подсчитывали количество лей коцитов, моноцитов, ней трофилов, лимфоцитов, Т-лимфоцитов, Т-хелперов, цитотоксических Т-клеток, регуляторных Т-клеток, NKT-лимфоцитов, NK-лимфоцитов; изучали фагоцитарную функцию ней трофилов и моноцитов, НСТ-редуцирующую и лизосомальную активность ней трофилов; определяли количество IgA, IgM, IgG, IL-4, IL-6, IL-8, IL-10, ЦИК. Результаты. На восьмые сутки после А-ФЛФ значимо повышалось число ней трофилов, показатели фагоцитоза ней трофилов и моноцитов, количество лимфоцитов, регуляторных Т-клеток, уровень IL-6 и IL-8; параллельно снижалось число CD11b+ NK-лимфоцитов, CD11b+ NKT-лимфоцитов, концентрации IgA, IgG, IL-10. На 24-е сутки количественно-функциональные показатели ней трофилов, общее число лимфоцитов, концентрации IgA и IgG не имели достоверных отличий от допроцедурных значений ; фагоцитарные параметры моноцитов, количество регуляторных Т-клеток, уровень IL-6 и IL-8 оставались достоверно выше, а число CD11b+ NK-лимфоцитов, CD11b+ NKT-лимфоцитов и IL-10, наоборот, значимо ниже исходного уровня. Обсуждение. Выявленные изменения показателей системного иммунитета после А-ФЛФ косвенно свидетельствуют как о прямом, так и о регуляторно-модулирующем влиянии иммунных факторов на восстановление и ремоделирование кожи после лазерного повреждения. Заключение. Процедура А-ФЛФ вызывает реакцию со стороны как клеточных, так и гуморальных факторов иммунной системы, преимущественно врожденного иммунитета.

Introduction. Ablative fractional laser photothermolysis (A-FLPh), used for rejuvenation of aging skin, is based on its controlled damage. Factors of the immune system are involved in the reparative regeneration processes triggered, which, in turn, is also subject to age-related remodeling or immunostaining. The aim of the work was to evaluate in dynamics the response of the immune system to the A-FLPh procedure performed for correction of age-associated facial skin changes. Materials and methods. The study included 25 women aged 42 to 55 years who underwent A-FLPh treatment of facial skin with an Erbium laser. The number of leukocytes, monocytes, neutrophils, lymphocytes, T-lymphocytes, T-helpers, cytotoxic T-cells, regulatory T-cells, NKT-lymphocytes, NK-lymphocytes were counted in the peripheral blood before, on the 8th and 24th after the procedure. We studied phagocytic function of neutrophils and monocytes, NBT-reducing and lysosomal activity of neutrophils; determined the amount of IgA, IgM, IgG, IL-4, IL-6, IL-8, IL-10, circulating immune complexes (CIC). Results. On the 8th day after A-FLPh, the number of neutrophils, neutrophils and monocytes phagocytosis, the number of lymphocytes, regulatory T-cells, IL-6 and IL-8 levels significantly increased; in parallel, the number of CD11b+ NK-lymphocytes, CD11b+ NKT-lymphocytes, IgA, IgG, IL-10 concentrations decreased. On the 24th day, quantitative functional indices of neutrophils, total number of lymphocytes, concentrations of IgA and IgG had no reliable difference from pre-procedure values, phagocytic parameters of monocytes, number of regulatory T-cells, IL-6 and IL-8 levels remained significantly higher, while the number of CD11b+ NK-lymphocytes, CD11b+ NKT-lymphocytes and IL-10, on the contrary, significantly lower than the initial level. Discussion. The revealed changes of systemic immunity indices after A-FLPh testify to both direct and regulatory-modulatory influence of immune factors on skin repair and remodeling after laser damage. Conclusion. The A-FLPh procedure induces a response from both cellular and humoral factors of the immune system, predominantly innate immunity.

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The authors declare that there are no conflicts of interest present.