urmjУральский медицинский журналUral Medical Journal2949-4389Ural State Medical University10.52420/2071-5943-2023-22-6-43-49urmj-1365Research ArticleСтатьиАнтитоксическая активность экстракта шишек ели обыкновенной при остром отравлении ацетатом свинца в экспериментеAntitoxic activity of spruce cone extract in acute lead acetate poisoning in experimenthttps://orcid.org/0000-0001-9464-1869ГуляевД. К.GuljaevD. K.

Дмитрий Константинович Гуляев, кандидат фармацевтических наук, доцент

кафедра фармакогнозии

Пермь

Dmitry K. Guljaev, PhD (Pharmacy), Associate Professor

Department of pharmacognosy

Perm

dkg2014@mail.ruТерехинГ. А.TerehinG. A.

Георгий Анатольевич Терехин, доктор медицинских наук, профессор, заведующий кафедрой

кафедрой экстремальной медицины и товароведения

Пермь

Georgy A. Terehin, Doctor of Science (Medicine), Professor, Head of the Department

Department of extreme medicine and commodity science

Perm

terehin-ga@yandex.ruhttps://orcid.org/0000-0001-9898-3479СемакинД. О.SemakinD. O.

Дмитрий Олегович Семакин, аспирант

кафедра фармакогнозии

Пермь

Dmitry O. Semakin, Postgraduate student

Department of pharmacognosy

Perm

dima98@gmail.comhttps://orcid.org/0000-0001-5193-3976БелоноговаВ. Д.BelonogovaV. D.

Валентина Дмитриевна Белоногова, доктор фармацевтических наук, доцент, заведующий кафедрой

кафедра фармакогнозии

Пермь

Valentina D. Belonogova, Doctor of Science (Pharmacy), Associate Professor, Head of the Department

Department of pharmacognosy

Perm

belonogova@pfa.ruПермская государственная фармацевтическая академияРоссияPerm State Pharmaceutical AcademyRussian Federation2023221220232264349Copyright © Гуляев Д.К., Терехин Г.А., Семакин Д.О., Белоногова В.Д., 20232023Гуляев Д.К., Терехин Г.А., Семакин Д.О., Белоногова В.Д.Guljaev D.K., Terehin G.A., Semakin D.O., Belonogova V.D.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://www.umjusmu.ru/jour/article/view/1365   Введение

   Введение. В настоящее время по всему миру продолжается загрязнение территорий свинцом. Актуальной задачей является разработка новых эффективных лекарственных средств для лечения отравлений свинцом.

   Цель работы – оценить эффективность применения сухого водного экстракта шишек ели обыкновенной при отравлении свинцом.

   Материалы и методы

   Материалы и методы. Эксперимент проведён на 24 белых беспородных крысах обоего пола. Животным исследуемых групп вводили водный раствор ацетета свинца. Животным третьей группы проводили лечение экстрактом шишек ели обыкновенной (экстракт) путем внутрижелудочного введения 3 раза в неделю в течение месяца. Животным четвертой группы проводили лечение препаратом полифеман путем внутрижелудочного введения 3 раза в неделю в течение месяца. Элементный состав образцов тканей животных исследуемых групп определяли на энергодисперсионном рентгенофлуоресцентном спектрометре марки QUANT’X. Для установления антиоксидантной активности экстракта использовали реакцию со свободным радикалом 2,2-дифенил-1-пикрилгидразилом.

   Результаты

   Результаты. В группах животных, которым вводили раствор ацетата свинца и проводили лечение экстрактом и препаратом сравнения полифепан, наблюдается снижение содержания свинца в костях итканях головного мозга по сравнению с содержанием у животных, которым вводили раствор ацетата свинца без лечения. Выявлено отсутствие влияния экстракта на моторную функцию кишечника. Приопределении антирадикальной активности установлено, что экстракт проявляет выраженную способность связывать свободные радикалы, сопоставимую с известным антиоксидантом рутином.

   Обсуждение

   Обсуждение. Применение экстракта позволяет эффективно выводить свинец и препятствовать его депонированию в костной ткани. Благодаря способности процианидинов проникать через гематоэнцефалический барьер экстракт эффективен при поражении свинцом головного мозга. Экстракт также будет эффективен для снятия окислительного стресса, возникающего при отравлении свинцом.

   Заключение

   Заключение. Сухой водный экстракт шишек ели обыкновенной проявляет способность снижать содержание свинца в костях и тканях головного мозга в сравнении с группой, не получавшей лечения на фоне отравления ацетатом свинца. Экстракт способен не только эффективно снижать содержание свинца в организме, но и бороться с последствиями отравления, благодаря выраженной антирадикальной активности.

   Introduction

   Introduction. Lead contamination is continuing worldwide. The development of new effective drugs for lead poisoning is an urgent task.

   The aim of the work is to evaluate the effectiveness of dry aqueous extract of spruce cones in lead poisoning.

   Materials and methods

   Materials and methods. The experiment was carried out on 24 white mongrel rats of both sexes. Animals of the studied groups were administered aqueous solution of lead acetate. The animals of the third group were treated with the extract of cones of common spruce (extract) by intragastric administration 3 times a week for a month. Animals of the fourth group were treated with polypheman by intragastric administration 3 times a week for a month. The elemental composition of tissue samples of animals of the studied groups was determined on energy dispersive X-ray fluorescence spectrometer QUANT’X. The free radical reaction with 2,2-diphenyl-1-picrylhydrazyl was used to determine the antioxidant activity of the extract.

   Results

   Results. In groups of animals injected with lead acetate solution and treated with the extract and the comparison preparation polyphepan, a decrease in lead content in bones and brain tissues was observed compared to the content in animals injected with lead acetate solution without treatment. No effect of the extract on the motor function of the intestine was revealed. When determining the antiradical activity, it was found that the extract showed a pronounced ability to bind free radicals, comparable to the known antioxidant rutin.

   Discussion

   Discussion. The use of the extract allows effective excretion of lead and prevents its deposition in bone tissue. Due to the ability of procyanidins to penetrate the blood-brain barrier, the extract is effective in brain damage caused by lead. The extract will also be effective in relieving oxidative stress resulting from lead poisoning.

   Conclusion

   Conclusion. The dry aqueous extract of cones of common spruce shows the ability to reduce the lead content in bones and brain tissues in comparison with the group that did not receive treatment for lead acetate poisoning. The extract is able not only to effectively reduce the lead content in the body, but also to fight the consequences of poisoning, due to its pronounced antiradical activity.

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The authors declare that there are no conflicts of interest present.