urmjУральский медицинский журналUral Medical Journal2949-4389Ural State Medical University10.52420/umj.23.3.46TRDJMNurmj-1534Research ArticleОригинальные статьи | Original articlesOriginal articlesИзменение химических параметров ротовой жидкости при возраст‑ассоциированных стоматологических заболеванияхMixed Saliva Chemical Parameters Changes in Age‑Related Oral Diseaseshttps://orcid.org/0000-0002-6092-3734КопенкинМ. А.KopenkinM. A.

Максим Александрович Копенкин — аспирант, младший научный сотрудник центральной научно-исследовательской лаборатории

Екатеринбург

Maksim A. Kopenkin — Postgraduate Student, Junior Researcher of the Central Research Laboratory

Ekaterinburg

maximkopenkin@yandex.ruhttps://orcid.org/0000-0002-4921-7222ПолушинаП. Г.PolushinaL. G.

Лариса Георгиевна Полушина — кандидат медицинских наук, старший научный сотрудник центральной научно-исследовательской лаборатории

Екатеринбург

Larisa G. Polushina — Candidate of Sciences (Medicine), Senior Researcher of the Central Research Laboratory

Ekaterinburg

polushina-larisa@bk.ruhttps://orcid.org/0000-0002-0296-8723СеменцоваЕ. А.SementsovaE. A.

Елена Анатольевна Семенцова — кандидат медицинских наук, доцент кафедры терапевтической стоматологии и пропедевтики стоматологических заболеваний

Екатеринбург

Elena A. Sementsova — Candidate of Sciences (Medicine), Associate Professor of the Department of Therapeutic Dentistry and Propaedeutics Dental Diseases

Ekaterinburg

vanevs@mail.ruhttps://orcid.org/0000-0002-8439-3272МандраЮ. В.MandraYu. V.

Юлия Владимировна Мандра — доктор медицинских наук, профессор кафедры терапевтической стоматологии и пропедевтики стоматологических заболеваний

Екатеринбург

Yulia V. Mandra — Doctor of Sciences (Medicine), Professor of the Department of Therapeutic Dentistry and Propaedeutics Dental Diseases

Ekaterinburg

jmandra@mail.ruhttps://orcid.org/0000-0003-0966-9571БазарныйВ. В.BazarnyiV. V.

Владимир Викторович Базарный — доктор медицинских наук, профессор, главный научный сотрудник центральной научно-исследовательской лаборатории

Екатеринбург

Vladimir V. Bazarnyi — Doctor of Sciences (Medicine), Professor, Chief Researcher of the Central Research Laboratory

Ekaterinburg

vlad-bazarny@yandex.ruУральский государственный медицинский университетРоссияUral State Medical UniversityRussian Federation2024110720242334658Copyright © Копенкин М.А., Полушина П.Г., Семенцова Е.А., Мандра Ю.В., Базарный В.В., 20242024Копенкин М.А., Полушина П.Г., Семенцова Е.А., Мандра Ю.В., Базарный В.В.Kopenkin M.A., Polushina L.G., Sementsova E.A., Mandra Y.V., Bazarnyi V.V.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://www.umjusmu.ru/jour/article/view/1534Введение

Введение. В пожилом и старческом возрастах увеличивается распространенность возраст-ассоциированных заболеваний полости рта, к которым относят хронический пародонтит и красный плоский лишай слизистой рта.

Цель исследования — оценить изменения биохимических параметров ротовой жидкости при возраст-ассоциированных заболеваниях полости рта с учетом пересчета концентрации аналитов на содержание общего белка. Материалы и методы. В исследовании приняло участие 86 человек пожилого возраста от 60 до 74 лет. Сформированы группы: пациенты с повышенной стираемостью зубов (n = 16); хроническим пародонтитом средней тяжести (n = 22); изменениями слизистой по типу красного плоского лишая (n = 28); здоровые добровольцы (n = 20). Для оценки физико-химических параметров ротовой жидкости применяли диагностические тест-полоски Siemens Multistix 10 SG (США), отражательный фотометр Siemens Clinitek Status+ (США), химический анализатор Mindray BS-240Pro (КНР). В работе проводилось определение 16 биохимических параметров и 4 расчетных индекса. Поскольку ротовая жидкость является нестандартизованной биожидкостью, непосредственно определяемые параметры пересчитывали путем деления на концентрацию общего белка пробы.

Результаты

Результаты. Установлено, что пациенты с красным плоским лишаем отличались от контрольной группы бÓльшим значением относительной плотности и высокими уровнями общего белка и кальция. Пациенты с хроническим пародонтитом имели более высокое содержание C-реактивного белка по сравнению с контролем. После приведения результатов по концентрации общего белка различия между группами по всем исследованным параметрам отсутствовали.

Обсуждение

Обсуждение. Данные соответствуют литературным источникам, описывающим изменения ротовой жидкости при возраст-ассоциированных заболеваниях, однако способы корректировки практически не использовались в рассмотренных работах.

Заключение

Заключение. Химические параметры ротовой жидкости меняются при возраст-ассоциированных заболеваниях рта. Необходим поиск оптимального метода корректировки, поскольку разведение и вязкость ротовой жидкости могут существенно влиять на результаты исследования.

Introduction

Introduction. The prevalence of oral cavity age-related diseases, such as chronic periodontitis (CP) and oral lichen planus (OLP) increases in the elderly and senile age people. The aim of the study was to evaluate changes in the biochemical parameters of mixed saliva in age-related oral diseases and evaluate impact of correction by total protein concentration.

Materials and methods

Materials and methods. The study included 86 elderly people from 60 to 74 years. They were divided into following groups: excessive attrition of teeth patients (n = 16); moderate CP patients (n = 22); OLP patients (n = 28); healthy peoples (n = 20). Reagent strips Siemens Multistix 10 SG (USA), analyzer Siemens Clinitek Status+ (USA), chemistry analyzer Mindray BS-240Pro (China) were used. In total, 16 biochemical markers and 4 calculated indices were determined. Saliva results were expressed without any correction, and corrected by total protein concentration.

Results

Results. It was found that OLP patients differed by a higher specific gravity and an increased level of total protein and calcium from the control group. CP patients had a higher level of c-reactive protein in compared to the healthy peoples. There were no differences between groups after correction.

Discussion

Discussion. The data corresponded to literature sources describing mixed saliva changes in age-related oral diseases. However, correction methods practically were not used in the considered works.

Conclusion

Conclusion. Mixed saliva chemical markers changed in age-related oral diseases. It is necessary to find the optimal correction method because dilution and viscosity can significantly affect mixed saliva research results.

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The authors declare that there are no conflicts of interest present.