urmjУральский медицинский журналUral Medical Journal2949-4389Ural State Medical University10.52420/umj.23.4.18BSVYLLurmj-1569Research ArticleОригинальные статьи | Original articlesOriginal articlesВозможности оценки основных диагностических маркеров при культивировании клеток люминального B подтипа рака молочной железыPossibilities of Assessing the Main Diagnostic Markers When Cultivating Luminal B Subtype Breast Cancer Cellshttps://orcid.org/0000-0002-7145-0733МогиленскихА. С.MogilenskikhA. S.

Анна Сергеевна Могиленских — научный сотрудник лаборатории клеточных культур, ассистент кафедры гистологии

Екатеринбург

Anna S. Mogilenskikh — Researcher of the Laboratory of Cell Cultures, Assistant of the Department of Histology

Ekaterinburg

annasajler@yandex.ruДерюгинМ. И.DeryuginM. I.

Михаил Игоревич Дерюгин — лаборант-исследователь патологоанатомического отделения, Институт медицинских клеточных технологий, ассистент кафедры гистологии

Екатеринбург

Michail I. Deryugin — Laboratory Technician and Researcher of the Pathoanatomical Department, Assistant of the Department of Histology

Ekaterinburg

mderyugin@mail.ruФадеевФ. А.FadeevF. A.

Фёдор Алексеевич Фадеев — кандидат биологических наук, доцент, заведующий лабораторией клеточных культур

Екатеринбург

Fedor A. Fadeev — Candidate of Sciences (Biology), Associate Professor, Head of the Laboratory of Cell Cultures

Ekaterinburg

fdf79@mail.ruДемидовС. М.DemidovS. M.

Сергей Михайлович Демидов — доктор медицинских наук, профессор, ведущий научный сотрудник патологоанатомического отделения, заведующий кафедрой онкологии и лучевой диагностики

Екатеринбург

Sergey M. Demidov — Doctor of Sciences (Medicine), Professor, Leading Researcher of the Pathoanatomical Department, Head of the Department of Oncology and Radiation Diagnostics

Ekaterinburg

professordemidov@mail.ruhttps://orcid.org/0000-0001-7064-0079СазоновС. В.SazonovS. V.

Сергей Владимирович Сазонов — доктор медицинских наук, профессор, заместитель главного врача по науке, заведующий патологоанатомическим отделением, заведующий кафедрой гистологии

Екатеринбург

Sergey V. Sazonov — Doctor of Sciences (Medicine), Professor, Deputy Chief Physician for Science, Head of the Pathoanatomical Department, Head of the Department of Histology

Ekaterinburg

prof-ssazonov@yandex.ruИнститут медицинских клеточных технологий; Уральский государственный медицинский университетРоссияInstitute of Medical Cellular Technologies; Ural State Medical UniversityRussian FederationИнститут медицинских клеточных технологийРоссияInstitute of Medical Cellular TechnologiesRussian Federation202406092024234Специальный выпуск «Онкология»18–28Copyright © Могиленских А.С., Дерюгин М.И., Фадеев Ф.А., Демидов С.М., Сазонов С.В., 20242024Могиленских А.С., Дерюгин М.И., Фадеев Ф.А., Демидов С.М., Сазонов С.В.Mogilenskikh A.S., Deryugin M.I., Fadeev F.A., Demidov S.M., Sazonov S.V.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://www.umjusmu.ru/jour/article/view/1569Введение

Введение. Несмотря на многочисленные проводимые исследования, посвященные комплексным сравнениям экспрессии мРНК, белков, мутаций в клеточных культурах и образцах тканей рака молочной железы, данных об их связи с изменениями рецепторного аппарата недостаточно.

Цель исследования — определение изменения уровней экспресии рецепторов в культуре клеток, полученной из образцов люминального В подтипа рака молочной железы, на протяжении пяти пассажей.

Материалы и методы

Материалы и методы. Произведен анализ эстрогена и Кi67, панцитокератина и виментина на протяжении пяти пассажей в клеточной культуре. Из части материала изготовлены парафиновые блоки для иммуно-гистохимического анализа, часть материала использовалась для получения клеточной суспензии для проведения проточной цитофлуориметрии.

Результаты

Результаты. Получена культура с высоким уровнем Ki-67 и экспрессии эстрогена на первых трех пассажах. Клетки сохраняли эпителиальную природу на протяжении всего культивирования.

Обсуждение

Обсуждение. Возможно, полученная культура может демонстрировать процессы, происходящие при региональном метастазировании. Об этом свидетельствует обнаружение опухолевых клеток, экспрессирующих виментин, с первого по четвертый пассаж. Изучение процессов, происходящих в моделях in vitro, поможет приблизиться к пониманию процессов в биологии рака молочной железы и выяснить природу гетерогенности этого заболевания.

Заключение

Заключение. Показана принципиальная возможность мониторинга основных параметров жизнедеятельности клеточной культуры люминального подтипа рака молочной железы, что позволяет создать эффективную модель наблюдения за культурами in vitro.

Introduction

Introduction. Despite extensive studies devoted to comprehensive comparisons of the expression of mRNA, proteins, mutations in cell cultures and tissue samples of breast cancer, there is insufficient data on the comparability of their receptor apparatus.

The purpose of the study was to determine changes in receptor expression levels in a cell culture obtained from a sample of the luminal B over five passages.

Materials and methods

Materials and methods. A cell culture was obtained. Estrogen and Ki‑67, pancytokeratin and vimentin were analyzed over five passages. Paraffin blocks were prepared from part of the material for immunohistochemical analysis.

Results and discussion

Results and discussion. A culture was obtained with high levels of Ki‑67 and estrogen expression during the first three passages. The cells maintained their epithelial nature throughout the culture. Perhaps this culture can demonstrate the processes occurring during regional metastasis. This is evidenced by the detection of tumor cells expressing vimentin from the first to the fourth passage. Thus, studying the processes occurring in in vitro models will help to come closer to understanding the processes in the biology of breast cancer and clarify the nature of the heterogeneity of this disease.

Conclusions

Conclusions. Studying the differences between primary culture cells and tumor tissue samples will provide an effective in vitro model.

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The authors declare that there are no conflicts of interest present.