urmjУральский медицинский журналUral Medical Journal2949-4389Ural State Medical University10.52420/umj.23.5.89QEHVCYurmj-1609Research ArticleОригинальные статьи | Original articlesOriginal articlesДинамика свободнорадикальных окислительных процессов в латентный период экспериментального метастазирования в печеньDynamics of Free Radical Oxidative Processes During the Latent Period of Experimental Metastasizing to the Liverhttps://orcid.org/0000-0003-3618-6890ФранциянцЕ. М.FrantsiyantsE. M.

Елена Михайловна Франциянц — доктор биологических наук, профессор, заместитель генерального директора по науке

Ростов‑на‑Дону

Elena М. Frantsiyants - Doctor of Sciences (Biology), Professor, Deputy General Director for Science

Rostov-on-Don

super.gormon@ya.ruhttps://orcid.org/0000-0002-4318-7587СуриковаЕ. И.SurikovaE. I.

Екатерина Игоревна Сурикова — кандидат биологических наук, старший научный сотрудник лаборатории изучения патогенеза злокачественных опухолей

Ростов‑на‑Дону

Ekaterina I. Surikova - Candidate of Sciences (Biology), Senior Researcher of the Laboratory of Malignant Tumor Pathogenesis Study

Rostov-on-Don

sunsur2000@mail.ruhttps://orcid.org/0000-0002-3972-2452КаплиеваИ. В.KaplievaI. V.

Ирина Викторовна Каплиева — доктор медицинских наук, заведующий лабораторией изучения патогенеза злокачественных опухолей

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Irina V. Kaplieva - Doctor of Sciences (Medicine), Head of the Laboratory of Malignant Tumor Pathogenesis Study

Rostov-on-Don

kaplirina@yandex.ruhttps://orcid.org/0000-0002-7395-3086НескубинаИ. В.NeskubinaI. V.

Ирина Валерьевна Нескубина — доктор биологических наук, старший научный сотрудник лаборатории изучения патогенеза злокачественных опухолей

Ростов‑на-Дону

Irina V. Neskubina - Doctor of Sciences (Biology), Senior Researcher of the Laboratory of Malignant Tumor Pathogenesis Study

Rostov-on-Don

neskubina.irina@mail.ruhttps://orcid.org/0009-0000-3589-2115ШакарянЭ. Г.ShakaryanE. G.

Элионора Григорьевна Шакарян — аспирант кафедры онкологии

Ростов‑на‑Дону

Elionora G. Shakaryan - Postgraduate Student of the Department of Oncology

Rostov-on-Don

elionora.shakaryan@mail.ruhttps://orcid.org/0000-0003-3998-8004СнежкоА. В.SnezhkoA. V.

Александр Владимирович Снежко — доктор медицинских наук, хирург отделения абдоминальной онкологии № 1; ассистент кафедры онкологии

Ростов‑на‑Дону

Alexander V. Snezhko - Doctor of Sciences (Medicine), Surgeon of the Department of Abdominal Oncology No. 1; Assistant of the Department of Oncology

Rostov-on-Don

snezhko.tanya@yandex.ruhttps://orcid.org/0000-0002-2302-8271БандовкинаВ. А.BandovkinaV. A.

Валерия Ахтямовна Бандовкина — доктор биологических наук, ведущий научный сотрудник лаборатории изучения патогенеза злокачественных опухолей

Ростов‑на‑Дону

Valeria A. Bandovkina - Doctor of Sciences (Biology), Leading Researcher of the Laboratory of Malignant Tumor Pathogenesis Study

Rostov-on-Don

valerryana@yandex.ruhttps://orcid.org/0000-0002-9749-2747ТрепитакиЛ. К.TrepitakiL. K.

Лидия Константиновна Трепитаки — кандидат биологических наук, научный сотрудник лаборатории изучения патогенеза злокачественных опухолей

Ростов‑на‑Дону

Lidia K. Trepitaki - Candidate of Sciences (Biology), Researcher of the Laboratory of Malignant Tumor Pathogenesis Study

Rostov-on-Don

legolab69@yandex.ruhttps://orcid.org/0000-0002-2674-9832ПогореловаЮ. А.PogorelovaYu. A.

Юлия Александровна Погорелова — кандидат биологических наук, старший научный сотрудник лаборатории изучения патогенеза злокачественных опухолей

Ростов‑на-Дону

Yulia А. Pogorelova - Candidate of Sciences (Biology), Senior Researcher of the Laboratory of Malignant Tumor Pathogenesis Study

Rostov-on-Don

https://orcid.org/0000-0003-2713-8598НемашкаловаЛ. А.NemashkalovaL. A.

Людмила Анатольевна Немашкалова — научный сотрудник лаборатории изучения патогенеза злокачественных опухолей

Ростов‑на‑Дону

Lyidmila A. Nemashkalova - Researcher of the Laboratory of Malignant Tumor Pathogenesis Study

Rostov-on-Don

nemalit@mail.ruhttps://orcid.org/0000-0001-5686-8659ЛесоваяН. С.LesovayaN. S.

Наталья Сергеевна Лесовая — младший научный сотрудник лаборатории изучения патогенеза злокачественных опухолей

Ростов‑на‑Дону

Natalia S. Lesovaya - Junior Researcher of the Laboratory of Malignant Tumor Pathogenesis Study

Rostov-on-Don

n.chugunova@bk.ruНациональный медицинский исследовательский центр онкологииРоссияNational Medical Research Centre for OncologyRussian FederationРостовский государственный медицинский университетРоссияRostov State Medical UniversityRussian FederationНациональный медицинский исследовательский центр онкологии; Ростовский государственный медицинский университетРоссияNational Medical Research Centre for Oncology; Rostov State Medical UniversityRussian Federation20241810202423589–103Copyright © Франциянц Е.М., Сурикова Е.И., Каплиева И.В., Нескубина И.В., Шакарян Э.Г., Снежко А.В., Бандовкина В.А., Трепитаки Л.К., Погорелова Ю.А., Немашкалова Л.А., Лесовая Н.С., 20242024Франциянц Е.М., Сурикова Е.И., Каплиева И.В., Нескубина И.В., Шакарян Э.Г., Снежко А.В., Бандовкина В.А., Трепитаки Л.К., Погорелова Ю.А., Немашкалова Л.А., Лесовая Н.С.Frantsiyants E.M., Surikova E.I., Kaplieva I.V., Neskubina I.V., Shakaryan E.G., Snezhko A.V., Bandovkina V.A., Trepitaki L.K., Pogorelova Y.A., Nemashkalova L.A., Lesovaya N.S.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://www.umjusmu.ru/jour/article/view/1609

Цель работы — изучение динамики содержания антиоксида нтных ферментов (супероксиддисмутазы 1 (СОД1), глутатионпероксидазы 1 (ГПО1) и глутатионредуктазы (ГР)) и продуктов перекисного окисления липидов (ПОЛ) (диеновых конъюгатов (ДК) и малонового диальдегида (МДА)) в селезенке и печени в латентный период роста и метастазирования экспериментальных опухолей.Материалы и методы. В экспериментах на 28 белых нелинейных крысах-самцах создана модель гематогенного метастазирования в печень путем перевивки саркомы 45 (С45) в селезенку, предварительно выведенную под кожу за 3 недели до этого. Ранее установлены характерные для модели сроки выхода опухоли в селезенке (5 недель) и визуализации метастазов в печени (7 недель) после трансплантации С45. В гомогенатах селезенки и печени методом иммуноферментного анализа определено содержание СОД1, ГПО1, ГР, МДА, биохимическим методом — содержание ДК в латентный период роста опухоли в селезенке и метастазирования ее в печень (1–2 недели после перевивки).Результаты. В указанные сроки описаны выраженные (в 1,5–5,2 раза, р < 0,050–0,001) изменения уровня исследованных факторов в сравнении с показателями у интактных животных и крыс с выведенной под кожу селезенкой. Полученные результаты свидетельствовали о параллельной активизации процессов ПОЛ и защитной антиоксидантной системы в селезенке (органе-опухоленосителе) в латентный период роста и метастазирования С45. Наблюдаемые в эти же сроки изменения исследованных показателей в печени (органе-мишени метастазирования) также указывали на активацию ПОЛ, но сопровождались выраженным снижением содержания ГР (в 5 раз, р < 0,001) без изменения уровня СОД1.Заключение. Результаты свидетельствуют о неполноценности антиоксидантной защиты и формировании прооксидантных условий в печени в латентный период роста опухоли, что может подготавливать почву для метастазирования.

Purpose — to investigate the dynamics of the content of antioxidant enzymes superoxide dismutase 1 (SOD1), glutathione peroxidase 1 (GPO1), glutathione reductase (GR) and lipid peroxidation products diene conjugates (DC), malondialdehyde (MDA) in the spleen and liver during the latent period of growth and metastasis of experimental tumor.Materials and methods. Using 28 white male rats, a model of hematogenous liver metastasis was created by transplanting sarcoma 45 cells (S45) into the spleen, previously lead out under the skin 3 weeks before. Previously, was determined that a tumor visualized in the spleen at 5 weeks, and liver metastases at 7 weeks after transplantation S45. Levels of SOD1, GPO1, GR and MDA were determined using ELISA and DC by biochemical method in spleen and liver homogenates during the latent period of tumor growth and metastasis (1–2 weeks post-transplantation).Results. Significant changes (1.5–5.2 times, р < 0.050–0.001) in studied factors levels were observed compared to intact rats and rats with the spleen lead out. Activation of lipid peroxidation and antioxidant system was noted in the spleen (tumor-carrying organ) during tumor growth and metastasis. At the same time, in the liver (the target organ of metastasis) observed also increased lipid peroxidation but simultaneously a pronounced decreased GR levels (5 times, p < 0.001) without affecting SOD1 levels.Conclusion. Liver tissue exhibited the inferiority of antioxidant protection and the formation of pro-oxidant condition during the latent period of tumor growth, which may prepare the soil for metastasis.

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The authors declare that there are no conflicts of interest present.