urmjУральский медицинский журналUral Medical Journal2949-4389Ural State Medical University10.52420/umj.24.4.116VMHCQIurmj-1939Research ArticleОригинальные статьи | Original articlesOriginal articlesОценка токсического влияния сплавов никелида титана с серебром на гематологический профиль лабораторных животныхThe Effect of Titanium Nickelide Alloys with Silver on the Hematological Profile of Laboratory Animalshttps://orcid.org/0000-0003-3157-4579ГордиенкоИ. И.GordienkoI. I.

Гордиенко Иван Иванович — кандидат медицинских наук, доцент, проректор по научно-исследовательской и инновационной деятельности, доцент кафедры детской хирургии, институт хирургии.

Екатеринбург

Ivan I. Gordienko — Candidate of Sciences (Medicine), Associate Professor, Vice-Rector for Research and Innovations, Associate Professor of the Department of Pediatric Surgery, Institute of Surgery, Ural State Medical University.

Ekaterinburg

ivan-gordienko@mail.ruhttps://orcid.org/0000-0002-4921-7222ПолушинаЛ. Г.PolushinaL. G.

Полушина Лариса Георгиевна — кандидат медицинских наук, заведующий центральной научно-исследовательской лабораторией, институт фундаментальной медицины.

Екатеринбург

Larisa G. Polushina — Candidate of Sciences (Medicine), Head of the Central Research Laboratory, Institute of Fundamental Medicine, Ural State Medical University.

Ekaterinburg

polushina-larisa@bk.ruhttps://orcid.org/0000-0001-9208-9417ДобринскаяМ. Н.DobrinskayaM. N.

Добринская Мария Николаевна — кандидат медицинских наук, доцент кафедры фармакологии и клинической фармакологии, институт клинической фармакологии и фармации.

Екатеринбург

Maria N. Dobrinskaya — Candidate of Sciences (Medicine), Associate Professor Department of Pharmacology and Clinical Pharmacology, Institute of Clinical Pharmacology and Pharmacy, Ural State Medical University.

Ekaterinburg

maria-nd@mail.ruhttps://orcid.org/0000-0003-4615-5270МарченкоЕ. С.MarchenkoE. S.

Марченко Екатерина Сергеевна — доктор физико-математических наук, доцент, заведующий лабораторией медицинских сплавов и имплантатов с памятью формы, Сибирский физико-технический институт.

Томск

Ekaterina S. Marchenko — Doctor of Sciences (Physics and Mathematics), Associate Professor, Head of the Laboratory of Medical Alloys and Implants with Shape Memory, Siberian Physical-Technical Institute, National Research Tomsk State University.

Tomsk

89138641814@mail.ruhttps://orcid.org/0000-0001-9050-3629ЦапН. А.TsapN. A.

Цап Наталья Александровна — доктор медицинских наук, профессор, заведующий кафедрой детской хирургии, институт хирургии.

Екатеринбург

Natalia A. Tsap — Doctor of Sciences (Medicine), Professor, Head of the Department of Pediatric Surgery, Institute of Surgery, Ural State Medical University.

Ekaterinburg

tsapna-ekat@rambler.ruhttps://orcid.org/0009-0002-0129-1244ЧерныйС. П.ChernyiiS. P.

Черный Степан Петрович — ассистент, аспирант кафедры детской хирургии, институт хирургии.

Екатеринбург

Stepan P. Chernyii — Assistant, Postgraduate Student of the Department of Pediatric Surgery, Institute of Surgery, Ural State Medical University.

Ekaterinburg

stechernyy@yandex.ruУральский государственный медицинский университетРоссияUral State Medical UniversityRussian FederationНациональный исследовательский Томский государственный университетРоссияNational Research Tomsk State UniversityRussian Federation202527082025244116–127Copyright © Гордиенко И.И., Полушина Л.Г., Добринская М.Н., Марченко Е.С., Цап Н.А., Черный С.П., 20252025Гордиенко И.И., Полушина Л.Г., Добринская М.Н., Марченко Е.С., Цап Н.А., Черный С.П.Gordienko I.I., Polushina L.G., Dobrinskaya M.N., Marchenko E.S., Tsap N.A., Chernyii S.P.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://www.umjusmu.ru/jour/article/view/1939Введение

Введение. Биомедицинские имплантаты находятся в стадии активных исследований. Никелид титана (NiTi) является уникальным сплавом, широко известным благодаря своим свойствам, таким как биосовместимость, прочность, пластичность. Несмотря на преимущества NiTi, его применение ограничено потенциальными рисками, связанными с аномальной реакцией тканей. В связи с этим усилия направлены на улучшение биосовместимости NiTi и снижение его токсичности.

Цель исследования — выявить потенциальные изменения гематологических показателей, оценить острую, подострую и хроническую токсичность, связанную с воздействием сплавов NiTi с включением серебра (Ag).

Материалы и методы

Материалы и методы. Исследование проводилось в рамках открытого рандомизированного контролируемого эксперимента длительностью 9 мес. Объектом изучения служили образцы порошка NiTi с добавлением Ag (образец 0,5 % Ag). В качестве контроля использовался порошок NiTi без добавления Ag. Проводилось исследование острой, подострой, субхронической и хронической системной токсичности, изучение гематологического профиля на лабораторных крысах.

Результаты

Результаты. При оценке состояния лабораторных животных после проведения теста по определению острой токсичности ни в одной группе за все время наблюдения не зарегистрированы гибель животных и неблагоприятные клинические признаки. Аналогично при изучении показателей общего анализа крови через 28 дней, 3, 6 и 9 мес. после внутримышечного подшивания образцов не выявлены воспалительные изменения, но отмечается противовоспалительная активность имплантата.

Заключение

Заключение. Оценка острой, подострой, субхронической и хронической токсичности NiTi не выявила гибели животных или неблагоприятных клинических признаков, указывающих на общее токсическое воздействие после подшивания образцов NiTi с Ag. В результате динамической оценки параметров общего анализа крови не выявлены изменения, характерные для токсического воздействия сплава на гемопоэз.

Introduction

Introduction. Biomedical implants in present time are under active research. Titanium nickelide (NiTi) is alloy widely uses for its properties. Despite the advantages of NiTi, its use is limited by the potential risks associated with abnormal tissue reaction. Therefore, significant efforts are being made to improve the biocompatibility of NiTi and reduce its toxicity.

The purpose of the study is to research potential changes in hematological parameters, to assess acute, subacute and chronic toxicity that may be associated with NiTi-silver (Ag) alloys.

Materials and methods

Materials and methods. The study was conducted as part of an open, randomized, controlled experiment lasting 9 months. The object of study was samples of NiTi powder with Ag addition. NiTi powder without the addition of Ag was used as a control. The study of acute, subacute, subchronic, chronic toxicity and hematological profile in particular, were conducted.

Results

Results. When assessing the condition of laboratory animals after conducting a test to determine acute systemic toxicity, animal deaths and adverse clinical signs were not recorded in any group of animals during the entire observation period. To study the potential for subacute toxicity, a general blood test was performed, and the potential anti-inflammatory activity of the implant was noted.

Conclusion

Conclusion. Assessment of acute, subacute, subchronic and chronic toxicity of NiTi revealed no signs of animal death or pronounced clinical symptoms indicating a general toxic effect after surgery. As a result of the dynamic assessment parameters of the blood, no changes characteristic of the toxic effect of the alloy on hematopoiesis were detected.

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The authors declare that there are no conflicts of interest present.