urmjУральский медицинский журналUral Medical Journal2949-4389Ural State Medical University10.52420/umj.24.5.50MOBCUFurmj-1971Research ArticleОригинальные статьи | Original articlesOriginal articlesОсобенности молекулярного взаимодействия карциномы легкого и туберкулеза в экспериментеFeatures of Molecular Interaction Between Lung Carcinoma and Tuberculosis In Vivohttps://orcid.org/0000-0002-1701-4180АгафоновГ. М.AgafonovG. M.

Агафонов Георгий Михайлович — торакальный хирург, стажер-исследователь, СПб НИИФ.

Санкт-Петербург

Georgiy M. Agafonov — Thoracic Surgeon, Trainee-Researcher, Saint Petersburg State Research Institute of Phthisiopulmonology.

Saint Petersburg

gm.agafonov@spbniif.ruhttps://orcid.org/0000-0002-2810-8852КудряшовГ. Г.KudriashovG. G.

Кудряшов Григорий Геннадьевич — кандидат медицинских наук, ведущий научный сотрудник, руководитель отдела пульмонологии и торакальной хирургии.

Санкт-Петербург

Grigorii G. Kudriashov — Candidate of Sciences (Medicine), Leading Researcher, Head of the Department of Pulmonology and Thoracic Surgery, Saint Petersburg State Research Institute of Phthisiopulmonology.

Saint Petersburg

dr.kudriashov.gg@yandex.comhttps://orcid.org/0000-0002-8698-7904КрыловаЮ. С.KrylovaJ. S.

Крылова Юлия Сергеевна — кандидат медицинских наук, патологоанатом, старший научный сотрудник центра молекулярной биомедицины.

Санкт-Петербург

Julia S. Krylova — Candidate of Sciences (Medicine), Pathologist, Senior Researcher of the Center of Molecular Biomedicine, Saint Petersburg State Research Institute of Phthisiopulmonology.

Saint Petersburg

info@spbniif.ruhttps://orcid.org/0000-0001-9518-2916ЗубареваТ. С.ZubarevaT. S.

Зубарева Татьяна Станиславовна — кандидат биологических наук, старший научный сотрудник центра молекулярной биомедицины.

Санкт-Петербург

Tatiana S. Zubareva — Candidate of Sciences (Biology), Senior Researcher of the Center of Molecular Biomedicine, Saint Petersburg State Research Institute of Phthisiopulmonology.

Saint Petersburg

tz6.6@bk.ruhttps://orcid.org/0000-0001-7302-5581КветнойИ. М.KvetnoyI. M.

Кветной Игорь Моисеевич — доктор медицинских наук, профессор, руководитель Центра молекулярной биомедицины, СПб НИИФ; профессор кафедры патологии, медицинский институт, СПбГУ.

Санкт-Петербург

Igor M. Kvetnoy — Doctor of Sciences (Medicine), Professor, Head of the Center of Molecular Biomedicine, Saint Petersburg State Research Institute of Phthisiopulmonology; Professor of the Department of Pathology, Institute of Medicine, Saint Petersburg State University.

Saint Petersburg

igor.kvetnoy@yandex.ruhttps://orcid.org/0000-0003-4385-9643ЯблонскийП. К.YablonskiyP. K.

Яблонский Пётр Казимирович — доктор медицинских наук, профессор, директор, СПб НИИФ; заведующий кафедрой госпитальной хирургии, медицинский институт, СПбГУ.

Санкт-Петербург

Piotr K. Yablonskii — Doctor of Sciences (Medicine), Professor, Director, Saint-Petersburg State Research Institute of Phthisiopulmonology; Head of the Department of Hospital Surgery, Institute of Medicine, Saint Petersburg State University.

Saint Petersburg

glhirurgb2@mail.ruСанкт-Петербургский научно-исследовательский институт фтизиопульмонологии; Санкт-Петербургский государственный университетРоссияSaint Petersburg State Research Institute of Phthisiopulmonology; Saint Petersburg State UniversityRussian FederationСанкт-Петербургский научно-исследовательский институт фтизиопульмонологииРоссияSaint Petersburg State Research Institute of PhthisiopulmonologyRussian Federation20250211202524550–63Copyright © Агафонов Г.М., Кудряшов Г.Г., Крылова Ю.С., Зубарева Т.С., Кветной И.М., Яблонский П.К., 20252025Агафонов Г.М., Кудряшов Г.Г., Крылова Ю.С., Зубарева Т.С., Кветной И.М., Яблонский П.К.Agafonov G.M., Kudriashov G.G., Krylova J.S., Zubareva T.S., Kvetnoy I.M., Yablonskiy P.K.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://www.umjusmu.ru/jour/article/view/1971Введение

Введение. Рак легкого и туберкулез возглавляют список социально значимых болезней во всем мире. Основы взаимодействия двух патологических процессов в настоящий момент до конца не изучены.

Цель исследования — изучить влияние туберкулеза легких на развитие злокачественной опухоли легкого.

Материалы и методы

Материалы и методы. Использована модель (патент RU 2800964 C1) эпидермоидной опухоли Льюис (LLC) и генерализованного туберкулеза (штаммы H37Rv и 5582) мышей линии C57BL/6. Сформировано 5 групп лабораторных животных: изолированный туберкулез (с различной чувствительностью), изолированная LLC и 2 группы сочетанной патологии с различной чувствительностью M. tuberculosis. Выполнено иммуногистохимическое исследование образцов легкого с определением относительной экспрессии TNF-α, PCNA и MMP-9.

Результаты

Результаты. Относительная экспрессия MMP-9 в группах сочетанной патологии статистически значимо ниже, чем в группе изолированной опухоли: LLC + H37Rv — 2,60; LLC + 5582–3,00; LLC — 8,90 (p = 0,043). Относительная экспрессия TNF-α не имела статистически значимых различий при сравнении групп сочетанной патологии и изолированной опухоли: LLC + H37Rv — 1,35; LLC + 5582–3,70; LLC — 1,70. Статистически значимо более высокий показатель экспрессии TNF-α наблюдался в группах мышей, инфицированных штаммом M. tuberculosis 5582. Относительная экспрессия PCNA статистически значимо ниже при сравнении групп сочетанной патологии и изолированной опухоли: LLC + H37Rv — 8,50; LLC + 5582–14,30; LLC — 36,45 (p = 0,012).

Обсуждение

Обсуждение. Полученные данные продемонстрировали, что инфицирование мышей с LLC штаммами M. tuberculosis приводит к подавлению опухоль-индуцированной экспрессии MMP-9 и снижению экспрессии PCNA, что может указывать на подавление процессов метастазирования и пролиферации опухолевых клеток. Уровень экспрессии TNF-α значимо не различался между группами, что не позволило сделать предположение о его влиянии на течение сочетанной патологии.

Заключение

Заключение. Полученные данные могут свидетельствовать об онкосупрессивном влиянии туберкулеза легких на развитие опухоли легкого.

Introduction

Introduction. The mechanisms of interaction between lung cancer and tuberculosis (TB) are not fully understood.

The aim of the study is to study the effect of pulmonary tuberculosis on the lung cancer.

Materials and methods

Materials and methods. The study utilized a model (Patent RU 2800964 C1) of isolated Lewis lung carcinoma (LLC) and generalized TB (H37Rv and 5582 strains) in C57BL/6 mice. Five groups of laboratory animals were formed: isolated tuberculosis (different M. tuberculosis susceptibility), isolated LLC, and 2 groups with combined pathology. An immunohistochemical analysis for TNF-α, PCNA, and MMP-9 was completed.

Results

Results. The relative expression of MMP-9 in the groups with combined pathology was significantly lower than in the isolated tumor group: LLC + H37Rv — 2.60; LLC + 5582–3.00; LLC — 8.90 (p = 0.043). The relative expression of TNF-α showed no statistically significant differences between the combined pathology groups and the isolated tumor group: LLC + H37Rv — 1.35; LLC + 5582–3.70; LLC — 1.70. A statistically significant increase in TNF-α expression was observed in mice infected with the drug-resistant M. tuberculosis strain (5582). The relative expression of PCNA was significantly lower in the combined pathology groups compared to the isolated tumor group: LLC + H37Rv — 8.50; LLC + 5582 — 14.30; LLC — 36.45 (p = 0.012).

Discussion

Discussion. Data demonstrated that TB led to the suppression of tumor-induced MMP-9 expression, reduction in PCNA expression. This may indicate the suppression of metastasisе, cell proliferation. The TNF-α expression level didn`t differ significantly between the groups.

Conclusion

Conclusion. The obtained data may suggest an oncosuppressive effect of tuberculosis on the lung tumors.

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The authors declare that there are no conflicts of interest present.