urmjУральский медицинский журналUral Medical Journal2949-4389Ural State Medical University10.52420/umj.25.3.23EOZTBWurmj-2228Research ArticleОригинальные статьи | Original articlesOriginal articlesКлинико-диагностическая ценность некоторых иммуноактивных пептидов при заболеваниях полости ртаThe Diagnostic Value of Certain Immunoactive Peptides in Oral Diseaseshttps://orcid.org/0000-0002-6092-3734КопенкинМ. А.KopenkinM. A.

Максим Александрович Копенкин — аспирант, ассистент кафедры патологической физиологии, институт фундаментальной медицины 

Екатеринбург 

Maksim A. Kopenkin — Postgraduate Student, Assistant of the Department of Pathological Physiology, Institute of Fundamental Medicine 

Ekaterinburg 

maximkopenkin@yandex.ruhttps://orcid.org/0009-0008-9706-8381МазмаеваА. М.MazmaevaA. M.

Аделина Марселевна Мазмаева — студент института клинической фармакологии и фармации 

Екатеринбург 

Adelina M. Mazmaeva — Specialist’s Degree Student of the Institute of Clinical Pharmacology and Pharmacy

Ekaterinburg 

tespeches@vk.comhttps://orcid.org/0009-0000-3814-7751НасоноваК. С.NasonovaK. S.

Карина Сергеевна Насонова — студент института клинической фармакологии и фармации 

Екатеринбург 

Karina S. Nasonova — Specialist’s Degree Student of the Institute of Clinical Pharmacology and Pharmacy 

Ekaterinburg 

karina.nasonova.05@bk.ruhttps://orcid.org/0000-0002-0296-8723СеменцоваЕ. А.SementsovaE. A.

Елена Анатольевна Семенцова — кандидат медицинских наук, доцент кафедры терапевтической стоматологии и пропедевтики стоматологических заболеваний, институт стоматологии 

Екатеринбург 

Elena A. Sementsova — Candidate of Sciences (Medicine), Associate Professor of the Department of Ther‑ apeutic Dentistry and Propaedeutics Dental Diseases, Institute of Dentistry 

Ekaterinburg 

vanevs@mail.ruhttps://orcid.org/0000-0001-8412-4315МаксимоваА. Ю.MaksimovaA. Yu.

Арина Юрьевна Максимова — кандидат медицинских наук, доцент кафедры медицинской микробиологии и клинической лабораторной диагностики, институт профилактической медицины 

Екатеринбург 

Arina Yu. Maksimova — Candidate of Sciences (Medicine), Associate Professor of the Department of Medi‑ cal Microbiology and Clinical Laboratory Diagnostics, Institute of Preventive Medicine

Ekaterinburg   

oreshek92@list.ruhttps://orcid.org/0000-0003-0966-9571БазарныйВ. В.BazarnyiV. V.

Владимир Викторович Базарный — доктор медицинских наук, профессор, главный научный сотрудник центральной научно-исследовательской лаборатории, институт фундаментальной медицины

Екатеринбург 

Vladimir V. Bazarnyi — Doctor of Sciences (Medicine), Professor, Chief Researcher of the Central Research Laboratory, Institute of Fundamental Medicine

Ekaterinburg 

vlad-bazarny@yandex.ruУральский государственный медицинский университетРоссияUral State Medical UniversityRussian Federation20260207202625323–33Copyright © Копенкин М.А., Мазмаева А.М., Насонова К.С., Семенцова Е.А., Максимова А.Ю., Базарный В.В., 20262026Копенкин М.А., Мазмаева А.М., Насонова К.С., Семенцова Е.А., Максимова А.Ю., Базарный В.В.Kopenkin M.A., Mazmaeva A.M., Nasonova K.S., Sementsova E.A., Maksimova A.Y., Bazarnyi V.V.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://www.umjusmu.ru/jour/article/view/2228Введение

Введение. Воспаление играет важную роль в патогенезе хронического пародонтита и красного плоского лишая слизистой оболочки полости рта. Определенный интерес в ранней диагностике, динамическом наблюдении, оценке эффективности лечения таких заболеваний представляют определяемые в ротовой жидкости как органоспецифическом субстрате полости рта медиаторы воспаления, в частности интерлейкин-6, интерлейкин-8 и Е-селектин.

Цель исследования — оценить клиническую информативность определения интерлейкина-6, интерлейкина-8 и Е-селектина в ротовой жидкости при хроническом пародонтите и красном плоском лишае, в т. ч. с использованием стандартизации результатов.

Материалы и методы

Материалы и методы. В исследование включено 43 человека разного возраста (63,0 [48,0; 71,0] года). Сформированы группы: пациенты с хроническим пародонтитом умеренной тяжести (n = 21); красным плоским лишаем слизистой оболочки рта (n = 7); здоровый контроль (n = 15). Для исследования ротовой жидкости использовался анализатор Luminex 200 (Th ermo Fisher Scientifi c, США); для определения в ротовой жидкости интерлейкина-6 — IL-6 Human ProcartaPlex Simplex Kit (Invitrogen, США), интерлейкина-8 — IL-8 (CXCL8) Human ProcartaPlex Simplex Kit (Invitrogen, США), Е-селектина — CD62E (E-selectin) Human ProcartaPlex Simplex Kit (Invitrogen, США). Данные стандартизированы путем деления на концентрацию общего белка.

Результаты

Результаты. Выявлено повышение абсолютной концентрации интерлейкина-8 у пациентов с хроническим пародонтитом относительно контроля (p = 0,019). ROC-анализ показал, что диагностическая точность определения интерлейкина-8 была умеренной (AUC = 0,790; p = 0,004). При точке отсечения ≥473,78 пг/мл диагностическая чувствительность составила 75,00 %, специфичность — 88,89 %. После стандартизации результатов различия отсутствовали.

Заключение

Заключение. Выявлено увеличение содержания интерлейкина-8 в ротовой жидкости при хроническом пародонтите, однако после стандартизации данных по содержанию общего белка подобные изменения отсутствовали. 

Introduction

Introduction. Inflammation plays a significant role in the pathogenesis of chronic periodontitis and oral lichen planus. Inflammatory mediators detected in mixed saliva, such as interleukin-6, interleukin-8, and E-selectin, are particularly important for early diagnosis, monitoring, and evaluating the effectiveness of treatments for these diseases.

The aim of the study was to evaluate the diagnostic value of absolute and corrected by total protein levels of interleukin-6, interleukin-8 and E-selectin in mixed saliva samples from patients with chronic periodontitis and oral lichen planus.

Materials and methods

Materials and methods. The study involved 43 people of different ages (63.0 [48.0; 71.0] years). Three groups were formed: patients with moderate chronic periodontitis (n = 21); patients with oral lichen planus (n = 7); control group (n = 15). The Luminex 200 analyzer (Thermo Fisher Scientific, USA) was used to evaluate levels of inteleukin-6 (IL-6 Human ProcartaPlex Simplex Kit, Invitrogen, USA), interleukin-8 (IL-8 (CXCL8) Human ProcartaPlex Simplex Kit, Invitrogen, USA) and E-selectin (CD62E (E-selectin) Human ProcartaPlex Simplex Kit, Invitrogen, USA) in mixed saliva.

Results

Results. The absolute concentration of interleukin-8 was significantly increased in patients with chronic periodontitis (p = 0.019). ROC analysis showed that the diagnostic accuracy of interleukin-8 measurement was moderate (AUC = 0.790; p = 0.004). Diagnostic sensitivity was 75.00 % and specificity was 88.89 % at the cut-off ≥473.78 pg/ml. There were no differences in the results after correction by total protein concentration.

Conclusion

Conclusion. The absolute level of interleukin-8 in mixed saliva was elevated in patients with chronic periodontitis, but there were no changes after correction by total protein concentration. 

интерлейкин-8интерлейкин-6Е-селектинхронический пародонтиткрасный плоский лишайротовая жидкостьinterleukin-8interleukin-6E-selectinchronic periodontitisoral lichen planusmixed salivaРабота проводилась в рамках государственного задания на научно-исследовательскую работу «Предикторы старения в полости рта и возможность их использования для персонификации стоматологического лечения» (регистрационный номер 121032300110-4).The work was carried out at the expense of the state task for the research work “Predictors of Aging in the Oral Cavity and the Possibility of Their Use for Personification of Dental Treatment” (registration number 121032300110-4).ReferencesFiorillo L. Oral health: The first step to well-being. Medicina. 2019;55(10):676. DOI: https://doi.org/10.3390/medicina55100676.Fiorillo L. Oral health: The first step to well-being. Medicina. 2019;55(10):676. DOI: https://doi.org/10.3390/medicina55100676.Spanemberg JC, Cardoso JA, Slob EMGB, Lopez-Lopez J. Quality of life related to oral health and its impact in adults. Journal of Stomatology, Oral and Maxillofacial Surgery. 2019;120(3):234–239. DOI: https://doi.org/10.1016/j.jormas.2019.02.004.Spanemberg JC, Cardoso JA, Slob EMGB, Lopez-Lopez J. Quality of life related to oral health and its impact in adults. Journal of Stomatology, Oral and Maxillofacial Surgery. 2019;120(3):234–239. DOI: https://doi.org/10.1016/j.jormas.2019.02.004.Trindade D, Carvalho R, Machado V, Chambrone L, Mendes JJ, Botelho J. Prevalence of periodontitis in dentate people between 2011 and 2020: A systematic review and meta-analysis of epidemiological studies. Journal of Clinical Periodontology. 2023;50(5):604–626. DOI: https://doi.org/10.1111/jcpe.13769.Trindade D, Carvalho R, Machado V, Chambrone L, Mendes JJ, Botelho J. Prevalence of periodontitis in dentate people between 2011 and 2020: A systematic review and meta-analysis of epidemiological studies. Journal of Clinical Periodontology. 2023;50(5):604–626. DOI: https://doi.org/10.1111/jcpe.13769.Ali Alftaikhah SA, Issrani R, Alnasser M, Almutairi HA, Khattak O, Iqbal A, et al. Salivary biomarkers in periodontitis: A scoping review. Cureus. 2023;15(12):e50207. DOI: https://doi.org/10.7759/cureus.50207.Ali Alftaikhah SA, Issrani R, Alnasser M, Almutairi HA, Khattak O, Iqbal A, et al. Salivary biomarkers in periodontitis: A scoping review. Cureus. 2023;15(12):e50207. DOI: https://doi.org/10.7759/cureus.50207.Hajishengallis G, Chavakis T, Lambris JD. Current understanding of periodontal disease pathogenesis and targets for host-modulation therapy. Periodontology 2000. 2020;84(1):14–34. DOI: https://doi.org/10.1111/prd.12331.Hajishengallis G, Chavakis T, Lambris JD. Current understanding of periodontal disease pathogenesis and targets for host-modulation therapy. Periodontology 2000. 2020;84(1):14–34. DOI: https://doi.org/10.1111/prd.12331.Sanadi RM, Khandekar PD, Chaudhari SR, Javali MA, Gurav NU. Association of periodontal disease with oral lichen planus: A systematic review and meta analysis. Journal of Oral and Maxillofacial Pathology. 2023;27(1):173–180. DOI: https://doi.org/10.4103/jomfp.jomfp_178_22.Sanadi RM, Khandekar PD, Chaudhari SR, Javali MA, Gurav NU. Association of periodontal disease with oral lichen planus: A systematic review and meta analysis. Journal of Oral and Maxillofacial Pathology. 2023;27(1):173–180. DOI: https://doi.org/10.4103/jomfp.jomfp_178_22.Li C, Tang X, Zheng X, Ge S, Wen H, Lin X, et al. Global prevalence and incidence estimates of oral lichen planus: A systematic review and meta-analysis. JAMA Dermatology. 2020;156(2):172–181. DOI: https://doi.org/10.1001/jamadermatol.2019.3797.Li C, Tang X, Zheng X, Ge S, Wen H, Lin X, et al. Global prevalence and incidence estimates of oral lichen planus: A systematic review and meta-analysis. JAMA Dermatology. 2020;156(2):172–181. DOI: https://doi.org/10.1001/jamadermatol.2019.3797.Zhu ZD, Ren XM, Zhou MM, Chen QM, Hua H, Li CL. Salivary cytokine profile in patients with oral lichen planus. Journal of Dental Sciences. 2022;17(1):100–105. DOI: https://doi.org/10.1016/j.jds.2021.06.013.Zhu ZD, Ren XM, Zhou MM, Chen QM, Hua H, Li CL. Salivary cytokine profile in patients with oral lichen planus. Journal of Dental Sciences. 2022;17(1):100–105. DOI: https://doi.org/10.1016/j.jds.2021.06.013.Vicic M, Hlaca N, Kastelan M, Brajac I, Sotosek V, Prpic Massari L. Comprehensive insight into lichen planus immunopathogenesis. International Journal of Molecular Sciences. 2023;24(3):3038. DOI: https://doi.org/10.3390/ijms24033038.Vicic M, Hlaca N, Kastelan M, Brajac I, Sotosek V, Prpic Massari L. Comprehensive insight into lichen planus immunopathogenesis. International Journal of Molecular Sciences. 2023;24(3):3038. DOI: https://doi.org/10.3390/ijms24033038.Kurago ZB. Etiology and pathogenesis of oral lichen planus: An overview. Oral Surgery, Oral Medicine, Oral Pathology and Oral Radiology. 2016;122(1):72–80. DOI: https://doi.org/doi:10.1016/j.oooo.2016.03.011.Kurago ZB. Etiology and pathogenesis of oral lichen planus: An overview. Oral Surgery, Oral Medicine, Oral Pathology and Oral Radiology. 2016;122(1):72–80. DOI: https://doi.org/doi:10.1016/j.oooo.2016.03.011.Bazarnyi VV, Kopenkin MA, Polushina LG, Sementsova EA, Mandra YuV. The diagnostic efficacy of determination some proteins of apoptosis mitochondrial pathway in saliva at age-related oral diseases. Klinicheskaya Laboratornaya Diagnostika (Russian Clinical Laboratory Diagnostics). 2023;68(9):518–526. (In Russ.). DOI: https://doi.org/10.51620/0869-2084-2023-68-9-518-526.Bazarnyi VV, Kopenkin MA, Polushina LG, Sementsova EA, Mandra YuV. The diagnostic efficacy of determination some proteins of apoptosis mitochondrial pathway in saliva at age-related oral diseases. Klinicheskaya Laboratornaya Diagnostika (Russian Clinical Laboratory Diagnostics). 2023;68(9):518–526. (In Russ.). DOI: https://doi.org/10.51620/0869-2084-2023-68-9-518-526.Kopenkin MA, Polushina LG, Sementsova EA, Mandra YuV, Bazarnyi VV. Mixed saliva chemical parameters changes in age-related oral diseases. Ural Medical Journal. 2024;23(3):46–58. (In Russ.). DOI: https://doi.org/10.52420/umj.23.3.46.Kopenkin MA, Polushina LG, Sementsova EA, Mandra YuV, Bazarnyi VV. Mixed saliva chemical parameters changes in age-related oral diseases. Ural Medical Journal. 2024;23(3):46–58. (In Russ.). DOI: https:// doi.org/10.52420/umj.23.3.46.Bazarnyi VV, Kopenkin MA, Polushina LG, Sementsova EA, Mandra YuV. Neurotrophins and vascular endothelial growth factor in oral fluid of elderly patients: Diagnostic value for chronic periodontitis and oral lichen planus. Pacific Medical Journal. 2024;(1):35–38. (In Russ.). DOI: https://doi.org/10.34215/1609-1175-2024-1-35-38.Bazarnyi VV, Kopenkin MA, Polushina LG, Sementsova EA, Mandra YuV. Neurotrophins and vascular endothelial growth factor in oral fluid of elderly patients: Diagnostic value for chronic periodontitis and oral lichen planus. Pacific Medical Journal. 2024;(1):35–38. (In Russ.). DOI: https://doi.org/10.34215/1609-1175-2024-1-35-38.Matsushima K, Yang D, Oppenheim JJ. Interleukin-8: An evolving chemokine. Cytokine. 2022;153:155828. DOI: https://doi.org/10.1016/j.cyto.2022.155828.Matsushima K, Yang D, Oppenheim JJ. Interleukin-8: An evolving chemokine. Cytokine. 2022;153:155828. DOI: https://doi.org/10.1016/j.cyto.2022.155828.Kany S, Vollrath JT, Relja B. Cytokines in inflammatory disease. International Journal of Molecular Sciences. 2019;20(23):6008. DOI: https://doi.org/10.3390/ijms20236008.Kany S, Vollrath JT, Relja B. Cytokines in inflammatory disease. International Journal of Molecular Sciences. 2019;20(23):6008. DOI: https://doi.org/10.3390/ijms20236008.Gomes PR, Rocha MD, Lira JA, Coelho FA, Alves EH, Nascimento HM, et al. Salivary biomarkers present in patients with periodontitis without clinical distinction: Findings from a meta-analysis. Medicina Oral, Patología Oral y Cirugía Bucal. 2023;28(5):e457–e466. DOI: https://doi.org/10.4317/medoral.25876.Gomes PR, Rocha MD, Lira JA, Coelho FA, Alves EH, Nascimento HM, et al. Salivary biomarkers present in patients with periodontitis without clinical distinction: Findings from a meta-analysis. Medicina Oral, Patología Oral y Cirugía Bucal. 2023;28(5):e457–e466. DOI: https://doi.org/10.4317/medoral.25876.Hajishengallis G. Periodontitis: From microbial immune subversion to systemic inflammation. Nature Reviews Immunology. 2015;15(1):30–44. DOI: https://doi.org/10.1038/nri3785.Hajishengallis G. Periodontitis: From microbial immune subversion to systemic inflammation. Nature Reviews Immunology. 2015;15(1):30–44. DOI: https://doi.org/10.1038/nri3785.Finoti LS, Nepomuceno R, Pigossi SC, Corbi SC, Secolin R, Scarel-Caminaga RM. Association between interleukin-8 levels and chronic periodontal disease: A PRISMA-compliant systematic review and meta-analysis. Medicine. 2017;96(22):e6932. DOI: https://doi.org/10.1097/MD.0000000000006932.Finoti LS, Nepomuceno R, Pigossi SC, Corbi SC, Secolin R, Scarel-Caminaga RM. Association between interleukin-8 levels and chronic periodontal disease: A PRISMA-compliant systematic review and meta-analysis. Medicine. 2017;96(22):e6932. DOI: https://doi.org/10.1097/MD.0000000000006932.El-Howati A, Thornhill MH, Colley HE, Murdoch C. Immune mechanisms in oral lichen planus. Oral Diseases. 2023;29(4):1400–1415. DOI: https://doi.org/10.1111/odi.14142.El-Howati A, Thornhill MH, Colley HE, Murdoch C. Immune mechanisms in oral lichen planus. Oral Diseases. 2023;29(4):1400–1415. DOI: https://doi.org/10.1111/odi.14142.Louisy A, Humbert E, Samimi M. Oral lichen planus: An update on diagnosis and management. American Journal of Clinical Dermatology. 2024;25(1):35–53. DOI: https://doi.org/10.1007/s40257-023-00814-3.Louisy A, Humbert E, Samimi M. Oral lichen planus: An update on diagnosis and management. American Journal of Clinical Dermatology. 2024;25(1):35–53. DOI: https://doi.org/10.1007/s40257-023-00814-3.Hirano T. IL-6 in inflammation, autoimmunity and cancer. International Immunology. 2021;33(3):127–148. DOI: https://doi.org/10.1093/intimm/dxaa078.Hirano T. IL-6 in inflammation, autoimmunity and cancer. International Immunology. 2021;33(3):127–148. DOI: https://doi.org/10.1093/intimm/dxaa078.Ali AAA, Ramadan AM, Mukhtar MM, Awadelkarim MOA, Ali DAA. Salivary IL-6 levels in chronic periodontitis patients with and without oral squamous cell carcinoma: A comparative cross-sectional study at khartoum dental teaching hospital, 2016–2017. The Saudi Dental Journal. 2025;37(7–9):65. DOI: https://doi.org/10.1007/s44445-025-00069-0.Ali AAA, Ramadan AM, Mukhtar MM, Awadelkarim MOA, Ali DAA. Salivary IL-6 levels in chronic periodontitis patients with and without oral squamous cell carcinoma: A comparative cross-sectional study at khartoum dental teaching hospital, 2016–2017. The Saudi Dental Journal. 2025;37(7–9):65. DOI: https://doi.org/10.1007/s44445-025-00069-0.Angiari S. Selectin-mediated leukocyte trafficking during the development of autoimmune disease. Autoimmunity Reviews. 2015;14(11):984–995. DOI: https://doi.org/10.1016/j.autrev.2015.06.006.Angiari S. Selectin-mediated leukocyte trafficking during the development of autoimmune disease. Autoimmunity Reviews. 2015;14(11):984–995. DOI: https://doi.org/10.1016/j.autrev.2015.06.006.Silva M, Videira PA, Sackstein R. E-selectin ligands in the human mononuclear phagocyte system: Implications for infection, inflammation, and immunotherapy. Frontiers in Immunology. 2018;8:1878. DOI: https://doi.org/10.3389/fimmu.2017.01878.Silva M, Videira PA, Sackstein R. E-selectin ligands in the human mononuclear phagocyte system: Implications for infection, inflammation, and immunotherapy. Frontiers in Immunology. 2018;8:1878. DOI: https://doi.org/10.3389/fimmu.2017.01878.Zhang J, Huang S, Zhu Z, Gatt A, Liu J. E-selectin in vascular pathophysiology. Frontiers in Immunology. 2024;15:1401399. DOI: https://doi.org/10.3389/fimmu.2024.1401399.Zhang J, Huang S, Zhu Z, Gatt A, Liu J. E-selectin in vascular pathophysiology. Frontiers in Immunology. 2024;15:1401399. DOI: https://doi.org/10.3389/fimmu.2024.1401399.Zhang J. Biomarkers of endothelial activation and dysfunction in cardiovascular diseases. Reviews in Cardiovascular Medicine. 2022;23(2):73. DOI: https://doi.org/10.31083/j.rcm2302073.Zhang J. Biomarkers of endothelial activation and dysfunction in cardiovascular diseases. Reviews in Cardiovascular Medicine. 2022;23(2):73. DOI: https://doi.org/10.31083/j.rcm2302073.

The authors declare that there are no conflicts of interest present.