Macroscopic evaluation of nasal mucosal regeneration after trauma (experimental study)
https://doi.org/10.52420/2071-5943-2023-22-4-69-76
Abstract
Introduction. Adhesions as a manifestation of pathological regeneration in the nasal cavity after surgical interventions is a rather pressing problem of modern medicine.
The aim of the study. was to evaluate the effectiveness of regenerative treatment regimens for nasal mucosal trauma under experimental conditions.
Materials and methods. A randomized experimental study was carried out on 480 male white rats, divided into six treatment-dependent groups. At control times (2, 5, 10, 14, 21, 30, 42, 60 days), 10 animals were each removed from the experiment.
Results. After nasal musocal trauma on the 2−5th day the processes of alteration prevailed, which were more pronounced in the group that did not receive anti-inflammatory treatment. In the group treated with a combination of the antioxidant “Demiphosphon” and the reparant “Derinat”, already from the 10th day there there was noted a restoration of the integrity of the mucous membrane of the wound edges, by the 60th day in all 10 (100 %) animals there was a complete closure of the defect. The incidence of adhesions in the group without treatment was 11.3 % with the use of reparative agents in the groups a statistically significant reduction of the adhesion process was observed.
Discussion. Other authors have described the effects of various drugs on wound healing and adhesions. The effectiveness of hyaluronic acid remains controversial; in the study presented, no nasal adhesions were detected when sodium hyaluronate (“Olifrin”) was used.
Conclusion. The best result was observed in the groups where the combination of the antioxidant “Demiphosphon” and the reparative agent “Derinat” was used: in addition to the absence of adhesions, earlier closure of the defect was noted. The inclusion of reparative agents in the treatment regimens increases the effectiveness of treatment by correcting the effects on reparative regeneration processes.
About the Author
I. Ye. Berest
St. Luke’s Lugansk State Medical University
Russian Federation
Russian Federation
Irina Ye. Berest, Ph.D. in medicine, Associate Professor, Department of Otorhinolaryngology and Ophthalmology,
Lugansk
References
1. Postalci L, Erdim I, Demirgil B et al. Postoperative rhinological complications after microscopic transnasal hypophysectomy. Turk Neurosurg. 2017;27(2):182−186. https://doi.org/10.5137/1019-5149.JTN.15754-15.1.
2. Svistushkin VM, Grachev NS, Mustafaev DM et al. Laser surgery for cicatricial obstructive diseases of the nasal cavity. Laser medicine = Lazernaja medicina. 2011;15(2):97. (In Russ.).
3. Alexanyan TA, Osipyan AA, Tovmasyan AS i dr. Adhesions of the nasal cavity. Russian Rhinology = Rossijskaja rinologija. 2021;29(4):216−221. (In Russ.). https://doi.org/10.17116/rosrino202129041216.
4. Dolci RL, Miyake MM, Tateno DA et al. Postoperative otorhinolaryngologic complications in transnasal endoscopic surgery to access the skull base. Braz J Otorhinolaryngol. 2016;83(3):349−355. https://doi.org/10.1016/j.bjorl.2016.04.020.
5. Proctor M, Proctor K, Shu XZ et al. Composition of hyaluronan affects wound healing in the rabbit maxillary sinus. Am J Rhinol. 2006;20(2):206−211. https://doi.org/10.1177/194589240602000218.
6. Khalmuratova R, Kim DW, Jeon SY. Effect of dexamethasone on wound healing of the septal mucosa in the rat. Am J Rhinol Allergy. 2011;25(3):112−116. https://doi.org/10.2500/ajra.2011.25.3595.
7. Trombitas V, Nagy A, Berce C et al. Effect of cigarette smoke on wound healing of the septal mucosa of the rat. BioMed Research International. 2016;1:1−11. https://doi.org/10.1155/2016/6958597.
8. Xu J, Zhang Q, Li Zh et al. Astragalus polysaccharides attenuate ovalbumin-induced allergic rhinitis in rats by inhibiting NLRP3 inflammasome activation and NOD2-mediated NF-κB activation. J Med Food. 2021;24(1):1−9. https://doi.org/10.1089/jmf.2020.4750.
9. Berest IE, Tananakina TP, Teleshova OV et al. Evaluating the developed model of experimental rhinitis in laboratory rats: pre-clinical experimental randomized study. Kuban Scientific Medical Bulletin = Kubanskij nauchnyj medicinskij vestnik. 2023;30(1):78–87. (In Russ.). https://doi.org/10.25207/1608-6228-2023-30-1-78-87.
10. Berest IE, Tananakina TP, Teleshova OV et al. Estimation of the method of extraction the ciliated epithelium of the nasal septum in laboratory rats. Crimean Journal of Experimental and Clinical Medicine = Krymskij zhurnal jeksperimental’noj i klinicheskoj mediciny. 2022;2(12):13−20. (In Russ.). https://doi.org/10.29039/2224-6444-2022-12-2-13-20.
11. Dubov VA. Comparative study of solcoseryl, mundizal and peridonton in the treatment of diseases of the oral mucosa. Russian Medical Journal = RMZh 2000;15:658. (In Russ.).
12. Myadelets OD, Lebedeva EI, Myadelets NY. Phosphatase-positive stem cells of rat skin while its posttraumatic regeneration in different conditions of wound inflicting. Bulletin of the Vitebsk State Medical University = Vestnik VGMU. 2018;17(3):44−57. (In Russ.). https://doi.org/10.22263/2312-4156.2018.3.44.
13. Porojskij SV, Firsova IV, Porojskaja AV, Makedonova JuA. Morphological characteristics of the process of reparative regeneration of the oral mucosa with various methods of its stimulation. Volgograd Scientific Medical Journal = Volgogradskij nauchno-medicinskij zhurnal. 2019;3:15−20. (In Russ.).
14. Belikov AV, Ermolaeva LA, Korzhevskij DJ et al. Research of oral mucosa regeneration after fractional treatment by diode laser with 980 nm wavelength. Scientific and Technical Journal of Information Technology, Mechanics and Optics = Nauchno-tehnicheskij vestnik informacionnyh tehnologij, mehaniki i optiki. 2018;18(5):719−726. (In Russ.). https://doi.org/10.17586/2226-1494-2018-18-5-719-726.
15. Pecherskij AV, Pecherskij VI, Shpilenja ES et al. Cicatrization and regeneration. Bulletin of the I.I. Mechnikov North-West State Medical University = Vestnik Severo-Zapadnogo gosudarstvennogo medicinskogo universiteta im. I.I. Mechnikova. 2015;3:73−81. (In Russ.).
16. Eremina MG, Eremin AV, Eldesbaeva JaS et al. Regenerative capabilities of the skin. Saratov Scientific Medical Journal = Saratovskij nauchno-medicinskij zhurnal. 2018;4:738−739. (In Russ.).
17. Chen D, Hou Q, Zhong L et al. Bioactive molecules for skin repair and regeneration: progress and perspectives. Stem Cells Int. 2019;2019:6789823. https://doi.org/10.1155/2019/6789823.
18. Choi KY, Cho SW, Choi J-J et al. Healing of the nasal septal mucosa in an experimental rabbit model of mucosal injury. World J Otorhinolaryngol Head Neck Surg. 2017;3(1):17−23. https://doi.org/10.1016/j.wjorl.2017.02.004.
19. Kunel’skaja NL, Carapkin GJu, Artem’ev ME et al. Conservative treatment of chemical damage of the nasal mucosa. Medical Council = Medicinskij sovet. 2013;3:63−64. (In Russ.).
20. Gouteva I, Shah-Hosseini K, Meiser P. Clinical efficacy of a spray containing hyaluronic acid and dexpanthenol after surgery in the nasal cavity (septoplasty, simple ethmoid sinus surgery, and turbinate surgery). J Allergy (Cairo). 2014;2014:635490. https://doi.org/10.1155/2014/635490.
21. Belvedere R, Novizio N, Eletto D et al. The procoagulant activity of Emoxilane®: a new appealing therapeutic use in epistaxis of the combination of sodium hyaluronate, silver salt, α-tocopherol and d-panthenol. Life (Basel). 2021;11(9):992. https://doi.org/10.3390/life11090992.
22. Gelardi M, Guglielmi AVN, De Candia N et al. Effect of sodium hyaluronate on mucociliary clearance after functional endoscopic sinus surgery. Eur Ann Allergy Clin Immunol. 2013;45(3):103−108.
23. Erdoğan O, İsmi O, Vayisoğlu Y et al. Comparison of saline, hyaluronic acid and xylitol nasal irrigation solutions after endoscopic sinus surgery: a prospective randomized study. KBB-Forum. 2023;22(1):057−062.
24. Casale M. New prospectives of Hyaluronic Acid in the upper airway chronic inflammation diseases. Glob J Oto. 2015;1(2):555−558. https://doi.org/10.19080/GJO.2015.01.555558.
25. Pignataro L, Marchisio P, Ibba T, Torretta S. Topically administered hyaluronic acid in the upper airway: A narrative review. Int J Immunopathol Pharmacol. 2018;32:2058738418766739. https://doi.org/10.1177/2058738418766739.
26. Budjakov SV. Immunocorrective efficacy of Derinat in maxillary sinusitis. Scientific Journal of Belgorod State University = Nauchnye vedomosti BelGU. 2010;22 (93):130−136. (In Russ.).
Review
For citations:
Berest IY. Macroscopic evaluation of nasal mucosal regeneration after trauma (experimental study). Ural Medical Journal. 2023;22(4):69-76. (In Russ.) https://doi.org/10.52420/2071-5943-2023-22-4-69-76
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