Morphological Study of the Mechanisms of Formation of Adhesions in the Abdominal Cavity and Exposure to Oxidized Dextran in an Experiment
https://doi.org/10.52420/umj.23.6.35
EDN: DPUAHC
Abstract
Introduction. Abdominal adhesions occur in diseases of the abdominal cavity and pelvic organs, reducing the quality of life of patients, leading to severe complications. The mechanisms of formation of adhesions are associated with inflammatory reaction and fibrosis, insufficient phagocytosis.
Materials and methods. In male Wistar rats (n = 20), the formation of adhesions was induced by laparotomy, drying and scarification of the peritoneum. Spontaneous formation of adhesions was researched in on the 7th and 21st days. Rats in group 1 (control), group 2 animals were intraperitoneally injected with aqueous solution of oxidized dextran. Researched the number of macrophages and neutrophils in inflammatory infiltrates, numerical density of cells, expressing TNFa, IL-1, E-cadherin, CK-18, Vimentin.
Results. In rats treated with oxidized dextran (group 2), the content of neutrophils on the 7th and 21st days in infiltrates was lower, and macrophages were greater, the numerical density of cells expressing IL-1 and TNFa was lower, comparatively with control group animals. The expression of Vimentin, E-cadherin, CK-18 was more pronounced in animals of the 1st group on the 7th day of the experiment. On the 21st day there were no differences in the expression of Vimentin, E-cadherin between the groups, and the expression of CK-18 was higher in group 2 rats.
Conclusion. Oxidized dextran affects the functional state of macrophages, prevents the expression of IL-1 and TNFa, promotes more effective phagocytosis, preventing the formation and fibrosis of adhesions, prevents the expression of markers of the mesenchymal to epithelial transition, contributing to the regeneration of the mesothelium.
About the Authors
M. A. Karpov
Federal Research Center of Fundamental and Translational Medicine; Novosibirsk State Medical University
Russian Federation
Competing Interests:
Russian Federation
Mikhail A. Karpov — Candidate of Sciences (Medicine), Leading Researcher of the Laboratory of the Structural Foundations of the Pathogenesis of Socially Significant Diseases, Research Institute of Experimental and Clinical Medicine, Federal Research Center of Fundamental and Translational Medicine; Associate Professor of the Department of Pathological Anatomy
Novosibirsk
Competing Interests:
The authors declare the absence of obvious or potential conflicts of interest.
A. P. Nadeev
Federal Research Center of Fundamental and Translational Medicine; Novosibirsk State Medical University
Russian Federation
Competing Interests:
Russian Federation
Aleksandr P. Nadeev — Doctor of Sciences (Medicine), Professor, Leading Researcher of the Laboratory of the Structural Foundations of the Pathogenesis of Socially Significant Diseases, Research Institute of Experimental and Clinical Medicine, Federal Research Center of Fundamental and Translational Medicine; Head of the Department of Pathological Anatomy, Novosibirsk State Medical University
Novosibirsk
Competing Interests:
The authors declare the absence of obvious or potential conflicts of interest.
V. A. Shkurupiy
Novosibirsk State Medical University
Russian Federation
Competing Interests:
Russian Federation
Viacheslav A. Shkurupiy — Doctor of Sciences (Medicine), Professor, Full Member (Academician) of the Russian Academy of Sciences, Professor of the of the Department of Pathological Anatomy, Novosibirsk State Medical University
Novosibirsk
Competing Interests:
The authors declare the absence of obvious or potential conflicts of interest.
S. V. Zalavina
Novosibirsk State Medical University
Russian Federation
Competing Interests:
Russian Federation
Svetlana V. Zalavina — Doctor of Sciences (Medicine), Professor, Head of the Department Histology, Embryology and Cytology
Novosibirsk
Competing Interests:
The authors declare the absence of obvious or potential conflicts of interest.
References
1. Wiseman D. Advances, retreats and challenges in adhesions research. Innova. 2016;(2):7–29. EDN: https://elibrary.ru/xyborf.
2. Samartsev V, Gavrilov V, Pushkarev B, Parshakov A, Kuznetsova MP, Kuznetsova M. Peritoneal adhesion: State of issue and modern methods of prevention. Perm Medical Journal. 2019;36(3):72–90. (In Russ.). DOI: https://doi.org/10.17816/pmj36372-90.
3. Eren EC, Basim P. Role of peripheral inflammatory biomarkers, transforming growth factor-beta and interleukin 6in predicting peritoneal adhesions following repeat cesarean delivery. Irish Journal of Medical Science. 2022;191(6):2697–2704. DOI: https://doi.org/10.1007/s11845-021-02878-8.
4. Coccolini F, Ansaloni L, Manfredi R, Campanati L, Poiasina E, Bertoli P, et al. Peritoneal adhesion index (PAI): Proposal of a score for the “ignored iceberg” of medicine and surgery. World Journal of Emergency Surgery. 2013;8:6. DOI: https://doi.org/10.1186/1749-7922-8-6.
5. Liu XR, Liu F, Li ZW, Liu XY, Zhang W, Peng D. The risk of postoperative complications is higher in stage I–III colorectal cancer patients with previous abdominal surgery: A propensity score matching analysis. Clinical and Translational Oncology. 2023;25(12):3471–3478. DOI: https://doi.org/10.1007/s12094-023-03210-9.
6. Kurtulus I, Basim S, Ozdenkaya Y. Can serum tumor necrosis factor-alpha predict peritoneal adhesions prior to secondary laparoscopic procedures? Journal of Visceral Surgery. 2023;160(4):261–268. DOI: https://doi.org/10.1016/j.jviscsurg.2022.12.007.
7. Atta HM, El-Rehany M, Roeb E, Abdel-Ghany H, Ramzy MM, Gaber SS. Mutant matrix metalloproteinase-9 reduces postoperative peritoneal adhesions in rats. International Journal of Surgery. 2016;26:58–63. DOI: https://doi.org/10.1016/j.ijsu.2015.12.065.
8. Ainal A, Saydam S. Effect of intraperitoneal and systemic sirolimus administration on postoperative peritoneal adhesions in rats. Journal of Basic and Clinical Health Sciences. 2021;5(3):195–200. DOI: https://doi.org/10.30621/jbachs.977476.
9. Gül A, Kotan Ç, Dilek I, Gül T, Tas A, Berktas M. Effects of methylene blue, indigo carmine solution and autologous erythrocyte suspension on formation of adhesions aſter injection into rats. Journal of Reproduction and Fertility. 2000;120(2):225–229. DOI: https://doi.org/10.1530/reprod/120.2.225.
10. Sahputra R, Dejyong K, Woolf AS, Mack M, Allen JE, Rückerl D, et al. Monocyte-derived peritoneal macrophages protect C57BL/6 mice against surgery-induced adhesions. Frontiers in Immunology. 2022;13:1000491. DOI: https://doi.org/10.3389/fimmu.2022.1000491.
11. Ito T, Shintani Y, Fields L, Shiraishi M, Podaru MN, Kainuma S, et al. Cell barrier function of resident peritoneal macrophages in post-operative adhesions. Nature Communications. 2021;12(1):2232. DOI: https://doi.org/10.1038/s41467-021-22536-y.
12. Strizova Z, Benesova I, Bartolini R, Novysedlak R, Cecrdlova E, Foley LK, et al. M1/M2 macrophages and their overlaps — myth or reality? Clinical Science. 2023;137(15):1067–1093. DOI: https://doi.org/10.1042/CS20220531.
13. Manda-Handzlik A, Cieloch A, Kuźmicka W, Mroczek A, Stelmaszczyk-Emmel A, Demkow U, et al. Secretomes of M1 and M2macrophages decrease the release of neutrophil extracellular traps. Scientific Reports. 2023;13:15633. DOI: https://doi.org/10.1038/s41598-023-42167-1.
14. Tang J, Xiang Z, Bernards MT, Chen S. Peritoneal adhesions: Occurrence, prevention and experimental models. Acta Biomaterialia. 2020;116:84–104. DOI: https://doi.org/10.1016/j.actbio.2020.08.036.
15. Zindel J, Mittner J, Bayer J, April-Monn SL, Kohler A, Nusse Y, et al. Intraperitoneal microbial contamination drives post-surgical peritoneal adhesions by mesothelial EGFR-signaling. Nature Communications. 2021; 12(1):7316. DOI: https://doi.org/10.1038/s41467-021-27612-x.
16. Yang L, Li Z, Chen Y, Chen F, Sun H, Zhao M, et al. Elucidating the novel mechanism of ligustrazine in preventing postoperative peritoneal adhesion formation. Oxidative Medicine and Cellular Longevity. 2022;2022:9226022. DOI: https://doi.org/10.1155/2022/9226022.
17. Shkurupiy VA, Kim LB, Kovner AV, Cherdantseva LA. Connective tissue and problems of its pathological conditions. Bulletin of Siberian Medicine. 2017;16(4):75–85. (In Russ.). DOI: https://doi.org/10.20538/1682-0363-2017-4-75-85.
18. Karpov MA, Shkurupy VA, Troitskii AV. The study of efficiency of the approach to prevent the adhesions in the abdominal cavity of rats. Bulletin of Experimental Biology and Medicine. 2021;171(4):416–420. DOI: https://doi.org/10.1007/s10517-021-05240-1.
19. Serebrennikova SN, Seminsky IZh, Guzovskaiia EV, Gutsol LO. Inflammation as a fundamental pathological process: Lecture 2 (cellular component). Baikal Medical Journal. 2023;2(2):65–76. (In Russ.). DOI: https://doi.org/10.57256/2949-0715-2023-2-65-76.
20. Othman A, Sekheri M, Filep JG. Roles of neutrophil granule proteins in orchestrating inflammation and immunity. The FEBS Journal. 2022;289(14):3932–3953. DOI: https://doi.org/10.1111/febs.15803.
21. Elrod J, Heuer A, Knopf J, Schoen J, Schönfeld L, Trochimiuk M, et al. Neutrophil extracellular traps and DNases orchestrate formation of peritoneal adhesions. iScience. 2023;26(12):108289. DOI: https://doi.org/10.1016/j.isci.2023.108289.
22. Puchkina G, Sulima A, Davidova A. Immunocompetent cells in the tissue of adhesives of patients with adhesion process in the small pelvis. Medical Herald of the South of Russia. 2021;12(3):72–77. (In Russ., Eng.). DOI: https://doi.org/10.21886/2219-8075-2021-12-3-72-77.
23. Mutsaers SE, Prêle CM, Pengelly S, Herrick SE. Mesothelial cells and peritoneal homeostasis. Fertility and Sterility. 2016;106(5):1018–1024. DOI: https://doi.org/10.1016/j.fertnstert.2016.09.005.
24. Semenov DA. The role of mesothelial cellsin compensatory-adaptive pleura reactionsin pathology (review of literature). Amur Medical Journal. 2019;(4):60–64. (In Russ.). DOI: https://doi.org/10.22448/AMJ.2019.4.60-64.
25. Sandoval P, Jiménez-Heffernan JA, Guerra-Azcona G, Pérez-Lozano ML, Rynne-Vidal Á, Albar-Vizcaíno P, et al. Mesothelial-to-mesenchymal transition in the pathogenesis of post-surgical peritoneal adhesions. The Journal of Pathology. 2016;239(1):48–59. DOI: https://doi.org/10.1002/path.4695.
Supplementary files
Review
For citations:
Karpov MA, Nadeev AP, Shkurupiy VA, Zalavina SV. Morphological Study of the Mechanisms of Formation of Adhesions in the Abdominal Cavity and Exposure to Oxidized Dextran in an Experiment. Ural Medical Journal. 2024;23(6):35–44. (In Russ.) https://doi.org/10.52420/umj.23.6.35. EDN: DPUAHC
Views:
171
Email this article 