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Experimental substantiation of the new method of immunological diagnosis of tuberculous pleurisy

https://doi.org/10.25694/URMJ.2018.05.56

Abstract

The current study was perfomed to investigate the representativeness of the basic and antigen induced levels of specific (IFN-y) and non-specific (IL-6) biomarkers for the diagnosis of tuberculous pleural effusion. Ninety-three samples of pleural fluid from patient with tuberculosis (TP) and non-tuberculosis (Non-TP) pleurisy were examined. Induction of the synthesis IFN-gamma by immunocompetent cells in pleural exudate was carried out by the recombinant protein CFP10-ESAT6 contained in the diagnosticum «DIASKINTEST» by our method (Patent RF, № 2664427; 2017). Serum and pleural fluid IFN-y and IL-6 levels were measured by enzyme-linked immunosorbent assay technique (enzyme-am-plified sensitivity immunoassay kits, Vector-Best reagent kit (Russia). Our results suggested that introduction of M. tuberculosis antigens into the pleural fluids samples of TP group caused a significant increase of the IFN-y levels compared to basic level, which were not observed in the Non-TP group. Detection of IFN-gamma antigen-induced level in pleural fluid is a promising method for the diagnosis of tuberculous pleurisy. We found no significant differences in the basic and antigen-induced levels of IL-6 in patients with TP and Non-TP pleurisy.

About the Authors

O. V. Kosareva
Уральский НИИ фтизиопульмонологии - филиал ФГБУ «НМИЦ ФПИ» Минздрава России
Russian Federation


S. N. Skornyakov
Уральский НИИ фтизиопульмонологии - филиал ФГБУ «НМИЦ ФПИ» Минздрава России
Russian Federation


S. V. Tsvirenko
ФГБОУ ВО «Уральский государственный медицинский университет» Минздрава России
Russian Federation


O. V. Fadina
Уральский НИИ фтизиопульмонологии - филиал ФГБУ «НМИЦ ФПИ» Минздрава России
Russian Federation


S. S. Karskanova
ФГБОУ ВО «Уральский государственный медицинский университет» Минздрава России; ГБУЗ СО «Противотуберкулезный диспансер»
Russian Federation


S. F. Melyakh
Уральский НИИ фтизиопульмонологии - филиал ФГБУ «НМИЦ ФПИ» Минздрава России
Russian Federation


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Review

For citations:


Kosareva OV, Skornyakov SN, Tsvirenko SV, Fadina OV, Karskanova SS, Melyakh SF. Experimental substantiation of the new method of immunological diagnosis of tuberculous pleurisy. Ural Medical Journal. 2018;(8):74-78. (In Russ.) https://doi.org/10.25694/URMJ.2018.05.56

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ISSN 2071-5943 (Print)
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