Use of stromal-vascular fraction from autological fat tissue under stressed urinary disease in men

The aim of the work is to develop a minimally invasive method for restoring the function of the sphincter of the urinary bladder using autologous fat in the stromal-vascular fraction (SVF). Materials and methods. Patients with a diagnosis of stress urinary incontinence of moderate severity were given a transurethral injection of an autologous SVF into the zone of the external sphincter of the bladder. Isolation of SVF was performed by a nonenzymatic method using tumescent lipoaspiration. The resulting material was sent to the laboratory for the study of cellular composition. Results. Based on the Pad test and ICIQ-SF questionnaires, QoL received the first positive clinical results and improved quality of life after 4 weeks. Conclusions. In this work, it is noted that transplantation of SVF from autologous adipose tissue appears to be an effective and safe method of treatment of patients with incontinence.

стромально-васкулярная фракция, стрессовое недержание мочи, трансплантация аутологичной жировой ткани, регенеративная медицина, stromal-vascular fraction, stress urinary incontinence, autologous adipose tissue transplantation, regenerative medicine

1. Dominik D., Anna L., Robert C. /Suction assisted liposuction does not impair the regenerative potential of adipose derived stem cells. //J Transl Med. 2016 May 6;14(1):126.

2. Stoltz J.F., Li Y.P. /Stem Cells and Regenerative Medicine: Myth or Reality of the 21th Century. //Stem Cells I nt. 2015; 2015: 734731.

3. Nie C., Yang D., Xu J. et al. /Locally administered adipose-derived stem cells accelerate wound healing through differentiation and vasculogenesis // Cell Transplant. 2011. Vol. 20. P. 205-216.

4. Gentile P., Orlandi A., Scioli M.G. et al. /Adipose-derived stromal vascular fraction cells and platelet-rich plasma: basic and clinical implications for tissue engineering therapies in regenerative surgery // Stem. Cells Transl. Med. 2012. Vol. 1. № 3. P. 230-236.

5. Bourin P., Bunnell B.A., Casteilla L., Dominici M., Katz A.J. /Stromal cells from the adipose tissue-derived stromal vascular fraction and culture expanded adipose tissue-derived stromal stem cells: a joint statement of the International Federation for Adipose Therapeutics and Science (IFATS) and the International Society for Cellular Therapy (ISCT). // Cytotherapy. - 2013. - Vol. 15. - № 6. - P. 641-648.

6. Basu J, Genheimer CW, Guthrie KI, Sangha N, Quinlan SF, et al. /Expansion of the human adipose-derived stromal vascular cell fraction yields a population of smooth muscle-like cells with markedly distinct phenotypic and functional properties relative to mesenchymal stem cells. //Tissue Eng Part C Methods 17: 843-860.

7. Laliberte M., Blelloch S., Ratanshi I., Safneck J., Buchel E. /Adipose-Derived Stromal Vascular Fraction Differentially Expands Breast Progenitors in Tissue Adjacent to Tumors Compared to Healthy Breast Tissue Sumanta Chatterjee// Plast Reconstr Surg. 2015 Oct;136(4):414e-25e.

8. You D., Jang M.J., Lee J., Suh N., Jeong I.G., et al. / Comparative analysis of periprostatic implantation and intracavernosal injection of human adipose tissue-derived stem cells for erectile function recovery in a rat model of cavernous nerve injury.// Prostate. 2013 Feb 15;73(3):278-86.

9. Bagno L.L., Werneck-de-Castro J.P., Oliveira P.F., Cunha-Abreu M.S., Rocha N.N., /Adipose-derived stromal cell therapy improves cardiac function after coronary occlusion in rats.// Cell Transplant. 2012;21(9):1985-96.

10. Brown, N. K., Z. Zhou, J. Zhang, R. Zeng, J. Wu, et al. /Perivascular adipose tissue in vascular function and disease: a review of current research and animal models.// Arterioscler. Thromb. Vasc. Biol. 34:16211630.

11. Farini A., Sitzia C., Erratico S., Meregalli M. / Clinical applications of mesenchymal stem cells in chronic diseases. // Stem Cells Int. - 2014. - Vol. 2014. - P. 306573.

12. Zhao W, Zhang C, Jin C, Zhang Z, et al./Periurethral injection of autologous adipose-derived stem cells with controlled-release nerve growth factor for the treatment of stress urinary incontinence in a rat model. //Eur Urol. 2010, 59: 155-163.

13. Wiafe B., Metcalfe P.D., Adesida A.B. /Stem Cell Therapy: Current Applications and Potential for Urology.// Curr Urol Rep. 2015 Nov;16(11).

14. Tokunori Yamamoto, Momokazu G., Ryohei H., Toriyama K. /Periurethral injection of autologous adipose-derived stem cells for the treatment of stress urinary incontinence in patients undergoing radical prostatectomy: Report of two initial cases. // International Journal of Urology (2010) 17, 75-82

15. Boissier R, Magalon J, Sabatier F, Veran J, Giraudo L, Giusiano S, /Histological and Urodynamic Effects of Autologous Stromal Vascular Fraction Extracted from Fat Tissue with Minimal Ex Vivo Manipulation in a Porcine Model of Intrinsic Sphincter Deficiency. //J Urol. 2016 Sep;196(3):934-42. doi: 10.1016/j. juro.2016.04.099. Epub 2016 Jun 2

16. Tran C., Damaser M.S./The potential role of stem cells in the treatment of urinary incontinence.//Ther Adv Urol. 2015Feb;7(1):22-40

17. Strasser H. /Stem-cell urological treatment was not carried out illegally.// Nature. 2008 Jun 26;453(7199):1177

18. Kollhoff DM, Cheng EY, Sharma AK/Urologic applications of engineered tissue.// Regen Med. 2011 Nov;6(6):757-65

19. Salomon L., Droupy S., Yiou R., Soulié M./Functional results and treatment of functional dysfunctions after radical prostatectomy.//Prog Urol. 2015 Nov;25(15).

20. Huixi Li, Guiting Lin./ Potential application of adipose tissue-derived stem cells for urological disease// Translational Andrology and Urology, Vol. 4, Supplement 1 (August 2015).

21. Rehman J, Traktuev D, Li J, Merfeld-Clauss S, Temm-Grove CJ, et al./Secretion of angiogenic and antiapoptotic factors by human adipose stromal cells.// Circulation 2004, 109: 1292-1298.

22. Meliga E, Strem BM, Duckers HJ, Serruys PW/Adiposederived cells. //Cell Transplant, 2007, 16: 963-970.

23. Banas A, Teratani T, Yamamoto Y, Tokuhara M, Takeshita F, et al./IFATS collection: in vivo therapeutic potential of human adipose tissue mesenchymal stem cells after transplantation into mice with liver injury. // Stem Cells, 2008, 26: 2705- 2712.

24. Ullah I., Subbarao R.B., Rho G.J. /Human mesenchymal stem cells - current trends and future prospective. // B iosci. Rep. 2015; 35(2): e00191.

25. Bara J.J., Richards R.G., Alini M. et al. /Concise review: B one marrow-derived mesenchymal stem cells change phenotype following in vitro culture: implications for basic research and the clinic. //Stem Cells 2014; 32(7): 1713-23.

26. Liao H.T., Chen C.T. /Osteogenic potential: Comparison between bone marrow and adiposederived mesenchymal stem cells. //World J.Stem Cells 2014; 6(3): 288-95.

27. Zuk P.A., Zhu M., Mizuno H. et al./Multilineage cells from human adipose tissue: implications for cell-based therapies.// Tissue Eng. 2001; 7(2): 211-28.

28. Zhu M., Heydarkhan-Hagvall S., Hedrick M. et al./ Manual isolation of adipose-derived stem cells from human lipoaspirates.// J. Vis. Exp. 2013; (79): e50585.

29. Qureshi A. T., Chen C., Shah F. et al./Human adipose-derived stromal stem cell isolation, culture, and osteogenic differentiation// Methods Enzymol. 2014; 538: 67-88.