Possibilities of using raman spectroscopy in diagnosis of prostate cancer in vitro
https://doi.org/10.25694/URMJ.201&09.29
Abstract
Purpose of the study. To assess the diagnostic capabilities of the Raman spectroscopy method in detecting prostate cancer. Materials and methods. Material selection: 13 samples of the prostate gland (histologically confirmed the diagnosis of "Benign prostatic hyperplasia” (BPH). 47 prostate tissue samples (histologically confirmed diagnosis "Prostate cancer." The samples were examined on the apparatus (HoribaScientific) .Configuration : wavelength 785 nm, grating 1200 gr / mm, 100% filter, confocal hole 300 pm. Integration time 50 s. Analysis of spectral data was carried out using Matlab software, Statistica, FreeSpectraBase, Sp ectralDatabase IndexBio-Rad. Results. Raman spectra of tissue fragments of BPH and PCa correspond to a range of 700-1800 cm-1. Samples with PCa have higher peaks compared to BPH samples at 1280 cm-1 (C-NH2, included in adenine, guanine, cytosine), 1323 cm-1 (CH3 / CH2 collagen), 1378 cm-1 (guanine, adenine, DNA), 1560 cm-1 (adenine and guanine). The spectra of the samples with BPH have higher peaks at 752 cm-1 (tyrosine), 1662 cm -1 (bond amides I: C = O proteins, C = C-bond of lipids). Conclusions. In the study of tissue samples with confirmed diagnoses of BPH and PCa, an increase in the intensity of Raman light scattering of DNA in tissues with confirmed PCa was noted, and a reverse decrease in the intensity of Raman scattering of DNA light in tissues with BPH. Raman spectroscopy showed spectral differences in the biochemical composition of tissues with BPH and tissues with PCa. In the future, this method of investigation can be used to develop a diagnostic algorithm for detecting prostate cancer.
About the Authors
V. N. Pavlov
ФГБОУ ВО «Башкирский Государственный Медицинский Университет»Минздрава России
Russian Federation
Russian Federation
A. R. Bilyalov
ФГБОУ ВО «Башкирский Государственный Медицинский Университет»Минздрава России
Russian Federation
Russian Federation
R. F. Gilmanova
ФГБОУ ВО «Башкирский Государственный Медицинский Университет»Минздрава России
Russian Federation
Russian Federation
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Review
For citations:
Pavlov VN, Bilyalov AR, Gilmanova RF. Possibilities of using raman spectroscopy in diagnosis of prostate cancer in vitro. Ural Medical Journal. 2018;(9):95-98. (In Russ.) https://doi.org/10.25694/URMJ.201&09.29
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