Effect of mechanoactivation of creatine and creatinine on buccal epithelium cells and erythrocytes electrokinetic activity

The influence of ball milling on the structure and biological activity of creatine and creatinine in relation to buccal epithelial cells and erythrocytes was studied. Mechanoactivation of creatine and creatinine does not change the type of crystal lattice, thus changing the lattice parameters. When creatine is mechanically activated, the steric position of the nitrogen-containing part of the molecule changes. The new structural state of the creatine molecule is unstable and breaks down after a few days in the powder sample, and the aqueous solution after 15 minutes. Ball milling leads to tautomeric transformation of a molecule of creatinine. It is revealed the interrelation of microelectrophoretic mobility with: 1) chemical structure of molecules: molecules with-N= and OH - groups and a large number of double bonds showed a slightly higher activity compared to tautomers with -NH and C=O groups; 2) charge state of nitrogen and oxygen atoms in molecules: increasing the charge on the nitrogen atom and lowering the charge on the oxygen atom is accompanied by an increase in the activity of cells in aqueous solutions of substances; 3) increased hydrophobicity and, consequently, lipo-philicity of molecules of the dissolved substance leads to an increase the cells activity in the solution; 4) it is possible that the greatest activity of cells is shown in solutions having electrical conductivity in the region of ~80-90 См; in solutions with lower (~25См) and higher (~120См) electrical conductivity the activity of cells is lower; it is possible that this effect is associated with the peculiarities of the interaction of charged functional groups of different nature with the cell membrane.

креатин, креатинин, механоактивация, микроэлектрофорез, эритроциты, эпителиоциты, creatine, creatinine, mechanoactivation, microelectrophoresis, erythrocytes, epi-thelial cells

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