The expression of the vascular endothelial growth factor and neoangiogenesis in experimental cerebral ischemia in conditions of combined use of erythropoietin and laser radiation

Abstract

The work was performed on 120 white non-linear rats. The cerebral cortex ischemia (CCI) was induced by diathermocoagulation of the pial vessels in the sensomotor zone of the cerebral cortex. Erythropoietin (EPO) was used intraperitoneally at a total dose of 15,000 IU/ kg, laser radiation (LR) - once to the area of the ischemic focus (970 nm, 2 min). The number of small blood vessels was counted and an expression of the vascular endothelial growth factor (VEGF) was determined in the ischemic focus. In case of CCI the expression of VEGF increases and the number of small blood vessels decreases in the ischemic focus on days 3, 7, 14, and 30. The use of EPO in CCI increases the number of small blood vessels, LR increases an expression of VEGF and the number of small blood vessels on the 3, 7, 14, 30 days of the experiment; the quantity of blood vessels is higher after exposure of LR as compared with the use of EPO. The maximal expression of VEGF and the number of small blood vessels in CCI are revealed after the combined use of EPO and LR.